The concentration of the antibody is lot specific and can be found on the certificate of analysis. (It is typically in the 1-2 mg/mL range).
F5766
Monoclonal Anti-Folic Acid antibody produced in mouse
clone VP-52, ascites fluid
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| Size/SKU | Availability | Price |
|---|---|---|
0.2 mL | Please contact Customer Service for Availability | CZK 13,800.00 |
0.5 mL | Please contact Customer Service for Availability | CZK 27,300.00 |
About This Item
Conjugate:
unconjugated
Clone:
VP-52, monoclonal
Application:
ELISA (i)
Citations:
8
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
VP-52, monoclonal
contains
15 mM sodium azide
technique(s)
indirect ELISA: 1:500
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Folic Acid (mouse IgG2b isotype) is derived from the hybridoma produced from the fusion of mouse myeloma cells and splenocytes of an immunized mouse. The normal range for folate in serum is 3-13 ng/ml.
Immunogen
5-Methyltetrahydrofolic acid (5MTHFA) conjugated to KLH
Application
Monoclonal Anti-Folic Acid antibody is suitable for use in western blot (diluted 1:1000).
Biochem/physiol Actions
Folic acid (folate) is a vitamin that regulates one-carbon transfer during the conversion of homocysteine to methionine. Methionine is a precursor of S-adenosylmethionine, which is the main methyl donor for methylation reactions. Thus, folic acid is essential for regulation of DNA methylations and for epigenetic modifications that modulate gene expression. Folate deficiency has been associated with a wide range of disorders including colorectal cancers, depression, neural tube defects and cerebral dysfunctions
Folic acid is essential for normal growth of mammalian cells. Folic acid deficiencies are common in pregnant women, alcoholics, those whose diets do not include raw fruits and vegetables, and people with structural or functional damage to the small intestine. Vitamin B12 and folic acid are metabolically interrelated.
Monoclonal Anti-Folic Acid antibody binds specifically to folate and detects an epitope present on both folic acid and its biologically active analog 5-methyltetrahydrofolic acid (5MTHFA). The product reacts with free folate, folate conjugated to a carrier such as KLH or BSA, and folic acid in human plasma or serum bound to the endogenous Folate Binder. The antibody does not cross-react with tetrahydrofolic acid, folinic acid or dihydrofolic acid.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| clone VP-52, monoclonal | clone FA-24, monoclonal | clone P6-38, monoclonal | clone HP-6017, monoclonal |
| antibody form ascites fluid | antibody form ascites fluid | antibody form ascites fluid | antibody form ascites fluid |
| isotype IgG2b | isotype IgG2b | isotype IgG1 | isotype IgG2a |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the concentration of Product No. F5766, Monoclonal Anti-Folic Acid antibody?
1 answer-
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What immunogen was used to generate Product No. F5766, Monoclonal Anti-Folic Acid antibody?
1 answer-
The immunogen used was 5-Methyltetrahydrofolic acid (sometimes called 5MTH folic acid) conjugated to keyhole limpet hemocyanin (KLH).
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the isotype of Product No. F5766, Monoclonal Anti-Folic Acid antibody?
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This antibody is mouse IgG2B.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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What is the name of the antibody clone for Product No. F5766, Monoclonal Anti-Folic Acid antibody?
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The clone is VP-52.
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Will Product No. F5766, Monoclonal Anti-Folic Acid antibody, work in Western blotting?
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The use of this antibody for western blotting has been reported in one study on Pseudomonas and tumor cells. See: Leamon, C.P., et al., Cytotoxicity of Folate-Pseudomonas Exotoxin Conjugates Toward Tumor Cells. J. Biol. Chem., 268(33), 24847-24854 (1993). The antibody was used at a 1 to 1000 dilution in Tris buffered saline (TBS) for 1 hour at 23°C.
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