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F4051

Proteasome Fraction II from rabbit

5 mg/mL, Reticulocyte cell extract, solution

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
MDL number:
MFCD03458197
Form:
solution
Quality level:
200

Quality Level

200

form

solution

concentration

5 mg/mL

storage temp.

−70°C

General description

Manufactured for Sigma by Boston Biochem., Inc.

Application

Proteasome Fraction II is ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radiolabeled or immunodetectable substrates. Isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the accumulation of ubiquitin protein conjugates. Typical assay concentration for FII is 0.5 - 4 mg/ml.

Biochem/physiol Actions

Essentially ubiquitin and ATP free. Contains E1, most E2s, UCHs, and the proteasome 20S and 26S isoforms.
Protein fraction of a cell extract that binds to anion-exchange resin.

Physical form

Solution in 25 mM HEPES, pH 7.6.

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This Item
U9257539162U9132
Proteasome Fraction II from rabbit 5 mg/mL, Reticulocyte cell extract, solution

Sigma-Aldrich

F4051

Proteasome Fraction II from rabbit

UbcH9 Conjugating Enzyme human ≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Sigma-Aldrich

U9257

UbcH9 Conjugating Enzyme human

Proteasome Inhibitor II The Proteasome Inhibitor II controls the biological activity of Proteasome. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

Sigma-Aldrich

539162

Proteasome Inhibitor II

Ubiquitin-Carrier Protein H7 human ≥95% (SDS-PAGE), recombinant, expressed in E. coli, liquid

Sigma-Aldrich

U9132

Ubiquitin-Carrier Protein H7 human

form

solution

form

buffered aqueous glycerol solution

form

lyophilized solid

form

liquid

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−70°C

concentration

5 mg/mL

concentration

-

concentration

-

concentration

-

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLBD8071
SLBD0228
SLBC3951
124K2710
014K2719

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A Hershko et al.
The Journal of biological chemistry, 261(26), 11992-11999 (1986-09-15)
In order to gain insight into the mechanisms that determine the selectivity of the ubiquitin proteolytic pathway, the protein substrate binding site of the ubiquitin-protein ligase system was identified and examined. Previous studies had shown that the ligase system consists
A Hershko et al.
The Journal of biological chemistry, 258(13), 8206-8214 (1983-07-10)
By affinity chromatography of a crude reticulocyte extract on ubiquitin-Sepharose, three enzymes required for the conjugation of ubiquitin with proteins have been isolated. One is the ubiquitin-activating enzyme (E1), which is covalently linked to the affinity column in the presence

Global Trade Item Number

SKUGTIN
F4051-5MG04061833261712

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