The density of the this product is not determined. The DNA concentration may range from 9.0 - 11.0 mg/mL. This value is lot-specific and reported in the Certificate of Analysis.
D7656
Deoxyribonucleic acid, single stranded from salmon testes
For hybridization
Synonym(s):
single-stranded template DNA
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1 ML
CZK 958.00
5 X 1 ML
CZK 6,370.00
About This Item
Assay:
9-11 mg/mL
Biological source:
fish testis
Form:
solution
Storage temp.:
−20°C
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biological source
fish testis
grade
Molecular Biology
assay
9-11 mg/mL
form
solution
concentration
10.0 ± 1.0 mg/mL in H2O
shipped in
dry ice
storage temp.
−20°C
Quality Level
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This Item | |||
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade for molecular biology, puriss. p.a. | grade for molecular biology |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 200 |
| form solution | form solution | form lyophilized powder | form solution |
| assay 9-11 mg/mL | assay 9-11 mg/mL (DNA concentration) | assay - | assay 9-11 mg/mL (DNA concentration) |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
General description
Deoxyribonucleic acid, single stranded from salmon testes is a ready-to-use solution of high quality, sonicated, single-stranded template DNA isolated from the testes of salmon.
Application
Deoxyribonucleic acid, single stranded from salmon testes has been used for/as:
- in situ hybridization histochemistry of rat brain[1], human kidney sections[2] and renal biopsy tissues[3]
- preparing standard curve of DNA[4]
- prehybridization solution in southern and northern hybridizations[5]
- dilution of the cDNA[6]
- fluorescent in-situ hybridization (FISH)[7]
- ChIP analysis[8]
- a standard to analyse the degraded nucleosides of genomic DNA by high performance liquid chromatography[9]
- a component of hybridization solution in FISH[10]
- a control in DNA-DNA hybridization experiment between various bacterial species[11]
- to precipitate probes in fluorescence in situ hybridization (FISH)[12][13][14]
For use as a blocking agent in Northern and Southern blotting.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Suitable for use as a blocking agent in Southern hybridizations.
Preparation Note
This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
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XY aneuploidy rate in sperm of two ?minor? breeds of cattle (Bos taurus) by using dual color fluorescent in situ hybridization (FISH)
Pauciullo, Alfredo, et al
Theriogenology, 688-695 (2010)
Rune Thomsen et al.
PloS one, 8(8), e72110-e72110 (2013-08-31)
The intermediate filament network of astrocytes includes Glial fibrillary acidic protein (Gfap) as a major component. Gfap mRNA is alternatively spliced resulting in generation of different protein isoforms where Gfapα is the most predominant isoform. The Gfapδ isoform is expressed
Jeffrey A Hodson et al.
Nucleic acids research, 31(21), e134-e134 (2003-10-25)
In this paper we report the construction of a Schizosaccharomyces pombe strain that facilitates analysis of replicating DNA. The strain co-expresses the Herpes simplex virus thymidine kinase gene (hsv-tk) and a human equilibrative nucleoside transporter (hENT1). The double integrant efficiently
Frequency of aneuploidy in in vitro-matured MII oocytes and corresponding first polar bodies in two dairy cattle (Bos taurus) breeds as determined by dual-color fluorescent in situ hybridization
Nicodemo, D., et al
Theriogenology, 523-529 (2010)
Similar rates of chromosomal aberrant secondary oocytes in two indigenous cattle (Bos taurus) breeds as determined by dual-color FISH
Pauciullo, Alfredo, et al
Theriogenology, 675-683 (2012)
Articles
Dostupné postupy, činidla a vybavení pro fluorescenční in situ hybridizaci (FISH).
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
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