N6019
Neutralizing Buffer
for Northern and Southern blotting, powder
Synonym(s):
Neutralizing Buffer for Blotting Techniques
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| Size/SKU | Availability | Price |
|---|---|---|
1 L | Please contact Customer Service for Availability | 3 140,00 CZK |
5 x 1 L | Please contact Customer Service for Availability | 11 400,00 CZK |
About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
3 140,00 CZK
Please contact Customer Service for Availability
grade
Molecular Biology
Quality Segment
form
powder
concentration
2.15-2.35 M chloride (by silver nitrate titration )
technique(s)
Southern blotting: suitable
foreign activity
DNase, RNase, none detected
storage temp.
room temp
General description
Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.
Application
Suitable for neutralizing the gel before transfer to membranes during Southern procedures.
Preparation Note
The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.
1 of 1
This Item | |||
|---|---|---|---|
| description for Northern and Southern blotting, powder | description liquid | description - | description histological tissue fixative |
| grade Molecular Biology | grade - | grade - | grade - |
| technique(s) Southern blotting: suitable | technique(s) - | technique(s) - | technique(s) - |
| concentration 2.15-2.35 M chloride (by silver nitrate titration ) | concentration - | concentration 0.5% (w/v, acetate buffer, pH 5.2. Preservative added.) | concentration 10% formalin (approx. 4% formaldehyde) |
| Quality Level 200 | Quality Level 200 | Quality Level 500 | Quality Level 200 |
| form powder | form liquid | form solution | form - |
| storage temp. room temp | storage temp. 2-8°C | storage temp. room temp | storage temp. room temp |
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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