C7673

Monoclonal Anti-CD14 antibody produced in mouse

clone UCHM-1, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-CD14
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: UCHM-1, monoclonal
• Application: FACS
• Citations: 10

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: UCHM-1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 55 kDa
• species reactivity: human
• technique(s): flow cytometry: 5 μL using 1 × 10⁶ cells
• isotype: IgG2a
• UniProt accession no.: P08571
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD14(929)

Description

General description

CD14 is a phosphoinositol-linked, single-chain, human cell surface glycoprotein antigen which is found on most human peripheral blood monocytes, granulocytes and dendritic reticulum cells, and some tissue macrophages. CD14 may also be present on B cells. Soluble forms of CD14 are present in serum and urine. The antigen functions as an acceptor for LPS-binding protein complexes.

Immunogen

human thymocytes followed by peripheral blood T cells.

Application

Monoclonal Anti-CD14 antibody produced in mouse is suitable for the following applications:

• Flow cytometry at a dilution of 5μL using 1 × 106 cells
• Immunofluorescence.
• Immunoblotting.

Biochem/physiol Actions

CD14 gene has an important role during signaling of the innate immune response and in the detection of inflammatory pathogens. It also plays an important role in recognizing tuberculosis (TB) disease and it can be used as a potential biomarker for predicting the prognosis of breast invasive ductal carcinoma with LN+ER/PR-Her²⁺. It is also associated with asthma, croup and allergy.

The epitope recognized by this clone is sensitive to routine formalin fixation and paraffin embedding. Cryostat sections, post-fixed in formalin can be stained.
3rd Workshop: code no. 203
4th Workshop: code no. M24

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable