C2969

Monoclonal Anti-I-CAM-1 (CD54) antibody produced in mouse

clone 8.4A6, purified immunoglobulin

• Synonym(s): Monoclonal Anti-CD54, Anti-I-CAM-1
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00240668
• Conjugate: unconjugated
• Clone: 8.4A6, monoclonal
• Application: ELISA (i), FACS, ICC, IHC (f)
• Citations: 1

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 8.4A6, monoclonal
• mol wt: antigen 85-110 kDa
• species reactivity: human, monkey
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (frozen sections): suitable, indirect ELISA: suitable
• isotype: IgG1
• UniProt accession no.: P05362
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ICAM1(3383)

Description

General description

CD54 (I-CAM-1) is a glycoprotein involved in mediation of intercellular adhesion. CD54 is expressed by activated endothelial cells and is also detected on cells of many other lineages and in plasma.

Immunogen

TNF-α activated human endothelial cells.

Biochem/physiol Actions

The antibody recognizes the human CD54 (I-CAM-1) surface antigen by flow cytometry, ELISA, immunocytochemistry, and immunohistochemistry. It inhibits human leukocyte adhesion to activated endothelial cells and stimulates homotypic aggregation of SKW3 cells. The epitope recognized by the antibody is localized in domain D2 of the CD54 molecule.
5th Workshop: code no. S 100

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4.

Preparation Note

Product filtered through a 0.2 μm filter

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves