These pelleted cells are prepared in 200 uL of RNAlater and are not intended for culture use. Should cells be required for continuous culture, see products listed as 'cryopreserved. Cryopreserved cell lines are prepared in an animal component free Keratinocyte Growth Medium.
C-14002
Normal Human Epidermal Keratinocytes (NHEK)
Adult, single donor, 1 million cells per pellet
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| Size/SKU | Availability | Price |
|---|---|---|
1 ea | Please contact Customer Service for Availability | 4 900,00 CZK |
About This Item
NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
human epidermis (adult)
Morphology:
(epidermal)
biological source
human epidermis (adult)
packaging
pkg of 1,000,000 cells
morphology
(epidermal)
application(s)
cell analysis (DNA, RNA, and protein)
shipped in
dry ice
storage temp.
−20°C
General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.
Application
Primary Normal Human Epidermal Keratinocytes (NHEK) are isolated from the epidermis of adult skin (different locations) from a single donor. They are the major cell type in the epidermis, making up about 90% of the cells.Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.Keratinocytes are also able to produce a variety of cytokines, growth factors, interleukins and complement factors. Therefore keratinocytes are important for wound healing, inflammation, and immune response.Normal Human Dermal Fibroblasts (NHDF) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.
Biochem/physiol Actions
Skin
Physical form
Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.
Preparation Note
Click here for more information.
Analysis Note
Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Other Notes
The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.
Legal Information
RNAlater is a registered trademark of Thermo Fisher Scientific, Inc.
Disclaimer
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
1 of 1
This Item | |||
|---|---|---|---|
| description Adult, single donor, 1 million cells per pellet | description Juvenile foreskin, pooled, 1 million cells per pellet | description Juvenile foreskin, single donor, 1 million cells per pellet | description Juvenile foreskin, single donor, 500,000 proliferating cells |
| morphology (epidermal) | morphology (epidermal) | morphology (epidermal) | morphology ( epidermal) |
| biological source human epidermis (adult) | biological source human foreskin (juvenile epidermis) | biological source human foreskin (juvenile epidermis) | biological source human foreskin (juvenile epidermis) |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in ambient |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. room temp |
| packaging pkg of 1,000,000 cells | packaging pkg of 1,000,000 cells | packaging pkg of 1,000,000 cells | packaging pkg of 500,000 cells |
| application(s) cell analysis (DNA, RNA, and protein) | application(s) cell analysis (DNA, RNA, and protein) | application(s) cell analysis (DNA, RNA, and protein) | application(s) - |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
7783-20-2
CAS No.
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What is the culture media these cells were grown in? Thanks!
1 answer
