C0979

Anti-Catalase antibody ,Mouse monoclonal

clone CAT-505, purified from hybridoma cell culture

• Synonym(s): Anti-CAT
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00802760
• Conjugate: unconjugated
• Clone: CAT-505, monoclonal
• Application: ARR, ELISA (i), ICC, IHC, WB
• Citations: 83

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: CAT-505, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 60 kDa
• species reactivity: human, mouse, bovine, rat
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using a whole extract of HepG2 cells
• isotype: IgG1
• UniProt accession no.: P04040
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CAT(847) ; mouse ... Cat(12359) ; rat ... Cat(24248)

Description

General description

Catalase is one of the most efficient and common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). In most mammalian cell types catalase is localized in peroxisomes which is a tertrameric haemin-enzyme consisting of 4 identical tetrahedrally arranged subunits of 60kDa. It contains four ferriprotoporphyrin groups per molecule having a molecular mass of 240kDa.

Immunogen

human erythrocyte catalase

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (5 papers)

Monoclonal Anti-Catalase antibody produced in mouse is suitable for the following:

• Immunocytochemistry
• Immunohistochemistry
• Indirect ELISA
• Microarray
• Western blotting at a concentration of 0.5-1μg/mL using whole extract of HepG2 cells
• Immunofluorescence

Biochem/physiol Actions

Catalase reacts with hydrogen peroxide, a reactive oxygen species, activating its decomposition into water and molecular oxygen and it reacts with hydrogen donors. It functions as a natural anti-oxidant, protecting cells against oxidative damage of proteins, lipids and nucleic acids. Catalase also plays a role in gene expression and apoptosis. Antioxidants have been identified in association with a variety of diverse cellular functions including growth control, proliferation, differentiation, immune response, tumor promotion, apoptosis and activation of viruses.

Physical form

Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable