C0871

Anti-Cullin 3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-CUL3
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03454920
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, IP, WB
• Citations: 12

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~80 kDa
• species reactivity: rat, mouse, human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysate of rat brain, indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells, western blot: 1-2 μg/mL using whole extract of HEK-293T cells expressing human Cullin 3
• UniProt accession no.: Q13618
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CUL3(8452) ; mouse ... Cul3(26554) ; rat ... Cul3(301555)

Description

General description

Cullin 3 is a member of the cullin family of E3 ubiquitin protein ligases. Cullin 3 forms the BTB-Cul3-Rbx1 (BCR) ubiquitin ligase complex that contains a RING-box protein, Rbx1, and a BTB containing protein. Rbx1 binds to the C-terminus of cullin 3 through its RING-type zinc finger domain to recruit a ubiquitin-conjugating enzyme. The BTB containing protein binds cullin 3 at its N-terminus and functions as a cullin specific adaptor protein.

Application

Anti-Cullin 3 antibody produced in rabbit has been used in

• western blotting[1]
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Cullin 3 (Cul3) acts as a key mediator of post-translational protein regulation. It functions as a scaffold protein that assemble multi-subunit ubiquitin ligase complexes which are responsible for the specific recognition and targeting of substrates for ubiquitin-dependent degradation by the 26S proteasome. Cul3 is required for degradation of the microtubule-severing protein MEI-1/katanin during transitioning from meiosis-to-mitosis phase. It forms a catalytically inactive BTB-Cul3-Rbx1 (BCR) ubiquitin ligase and becomes functional upon covalent attachment of the ubiquitin homologue neural-precursor-cell-expressed. BCR ubiquitin ligase down regulated 8 (Nedd8) near the C terminus of Cul3.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
• wgk: nwg
• Storage Class: 12 - Non Combustible Liquids