The specific site of biotin attachment on the IgG molecule has not been determined.
Additionally, the biotinylation procedure is proprietary and, therefore, cannot be disclosed.
B9183
Anti-HA-Biotin antibody, Mouse monoclonal
clone HA-7, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-HA, Monoclonal Anti-HA-Biotin antibody produced in mouse, Anti-HA, Anti-Influenza Hemagglutinin
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| Size/SKU | Availability | Price |
|---|---|---|
100 μg | Please contact Customer Service for Availability | CZK 11,700.00 |
About This Item
Conjugate:
biotin conjugate
Clone:
HA-7, monoclonal
Application:
WB CL
Citations:
7
CZK 11,700.00
Please contact Customer Service for Availability
biological source
mouse
Quality Segment
conjugate
biotin conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
buffered aqueous solution
concentration
~1 mg/mL
technique(s)
western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts.
shipped in
dry ice
storage temp.
2-8°C
General description
The Monoclonal Anti-HA Biotin Conjugate antibody recognizes the HA tag sequence on the HA tagged fusion proteins when expressed at the N or C terminal to the fusion protein. This antibody is derived from the HA-7 hybridoma, which is produced from the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH. Ascites fluid collected from the HA-7 hybridoma is then conjugated to biotin.
Immunogen
synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH
Application
Antibody suitable for immunoblotting.
Biochem/physiol Actions
The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
1 of 1
This Item | |||
|---|---|---|---|
| antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form ascites fluid | antibody form affinity isolated antibody |
| clone HA-7, monoclonal | clone HA-7, monoclonal | clone HA-7, monoclonal | clone polyclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
| conjugate biotin conjugate | conjugate TRITC conjugate | conjugate unconjugated | conjugate unconjugated |
| technique(s) western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts. | technique(s) direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins | technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein | technique(s) immunoprecipitation (IP): 2.5-4 μg/test using HA-tagged fusion protein from cell lysates, indirect immunofluorescence: 10-20 μg/mL using HA-tagged fusion protein transfected cells, western blot: 0.5-0.8 μg/mL using HA-tagged fusion protein transfected cell extracts |
| form buffered aqueous solution | form buffered aqueous solution | form - | form buffered aqueous solution |
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Where is the biotin located on the IgG? Additionally, what protocol or method did you follow to biotinylate it?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
1 answer-
Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
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