B7151

Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat

Name: Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162621

Conjugate:

biotin conjugate

Clone:

polyclonal

Application:

ELISA (d), IHC (p), WB

Citations:

This product is discontinued. Contact Technical Servicefor assistance.

Key Documents

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biological source

goat

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:200,000-1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:400,000-1:800,000 using total cell extract of HeLa cells

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

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Secondary Antibodies

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Show Differences

1 of 4

This Item	B0529	B7401	B8895
Sigma-Aldrich B7151 Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat	Sigma-Aldrich B0529 Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat	Sigma-Aldrich B7401 Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat	Sigma-Aldrich B8895 Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat
biological source goat	biological source goat	biological source goat	biological source goat
conjugate biotin conjugate	conjugate biotin conjugate	conjugate biotin conjugate	conjugate biotin conjugate
technique(s) direct ELISA: 1:200,000-1:300,000, western blot: 1:400,000-1:800,000 using total cell extract of HeLa cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200	technique(s) direct ELISA: 1:200,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot: 1:300,000-1:600,000 using using Hela cells extract	technique(s) direct ELISA: 1:160,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, western blot (chemiluminescent): 1:300,000 using total cell extract of HeLa cells (5-10 μg/well)	technique(s) direct ELISA: 1:200,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800, western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution

General description

IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. IgG is found in four subclasses IgG1, IgG2, IgG3 and IgG4. IgG has a monomeric structure.

The product binds to all mouse Igs

Application

Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat was used in immunohistochemistry of lateral and medial menisci of rabbits and human adipose-derived stem cells.

Biochem/physiol Actions

IgG (immunoglobulin G) antibody participates in complement fixation and opsonization.

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Mouse IgG (Fab specific) antibody allows for the binding of various labels such as avidin or streptavidin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000453482

0000140665

0000312830

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Anik Chevrier et al.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 27(9), 1197-1203 (2009-02-27)

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