A9776

α-Actinin from chicken gizzard

~80% α-actinin basis (SDS-PAGE), ammonium sulfate suspension

• CAS Number: 11003-00-2
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00130473
• Form: ammonium sulfate suspension
• Assay: ~80% α-actinin basis (SDS-PAGE)
• Biological source: chicken gizzard
• Mol wt: 100 kDa

Properties

• biological source: chicken gizzard
• assay: ~80% α-actinin basis (SDS-PAGE)
• form: ammonium sulfate suspension
• mol wt: 100 kDa
• UniProt accession no.: P05094, P12814, P20111, Q7TPR4, Q90734, Q9Z1P2
• storage temp.: 2-8°C
• Gene Information: chicken ... ACTN2(396263), ACTN4(396024), RCJMB04_23c5(373918) ; human ... ACTN1(87) ; mouse ... ACTN1(109711) ; rat ... ACTN1(81634)

Description

General description

α-Actinin exists as a rod-shaped antiparallel dimer with two elongated subunits. The domain region contains an N-terminal actin-binding domain with tandem calponin homology domains, a central tandem 3-helix motifs, and EF-hand motifs at C-terminus. It belongs to the spectrin superfamily and is localized in actin structures.[1]

Application

α-Actinin from chicken gizzard has been used:

• as an antigen to coat plates for the capture of anti-actinin antibody using enzyme-linked immunosorbent assay (ELISA) from tumor cell line clones[2]
• in in vitro motility experiments to test its effect on actin filament movement[3]
• to coat cantilevers for strengthening fluorescently labeled actin filaments in force measurement studies[4]

Biochem/physiol Actions

α-Actinin binds actin and has the ability to crosslink actin cytoskeleton. It also plays a key role in the structural maintenance of the Z-disk of striated muscle.[1]

Packaging

Package size based on protein content

Physical form

Suspension in 2 M (NH₄)₂SO₄ containing 20 mM Tris acetate, pH 7.6, 20 mM sodium chloride, 0.1 mM EDTA, 15 mM β-mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride

Preparation Note

Alpha-actinin ammonium sulfate suspension should be mixed sufficiently and further diluted prior to use. Protein precipitate will be present in solution with high protein concentrations.

Prepared using a modification of the procedure of Neidel, J.E. and Cuatrecasas, P., Biochem. Biophys. Res. Commun., 91, 152 (1979).

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)