A8452

Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse

ascites fluid, clone C3

• Synonym(s): Afp Antibody, Afp Antibody - Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse, Alpha Fetoprotein Antibody
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162111
• Conjugate: unconjugated
• Clone: C3, monoclonal
• Application: DB, ELISA (i), IHC (p)
• Citations: 92

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: C3, monoclonal
• contains: 15 mM sodium azide
• species reactivity: pig, canine, human
• should not react with: mouse, cat, rat, bovine
• technique(s): dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human fetal liver, indirect ELISA: suitable
• isotype: IgG2a
• UniProt accession no.: P02771
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... AFP(174)

Description

General description

α-Fetoprotein (AFP) is a one of the main glycoprotein present in the fetal liver, gastrointestinal tract, and yolk sac. AFP levels in amniotic fluid have been used as biomarkers for anencephaly and spina bifida.

AFP is a plasma protein found highly expressed in the human fetus where it functions to block the transport of estradiol across the placenta.

Monoclonal Anti-α-Fetoprotein (AFP) (mouse IgG2a isotype) is derived from the C3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human α-fetoprotein.

Immunogen

human α-fetoprotein

Application

Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• dot blot
• immunocytochemistry
• immunofluorescence
• immunocytochemistry

Mouse Monoclonal Anti-α-Fetoprotein has been used for immunocytochemistry[1]. The product can also be used for dot blot and indirect ELISA.

Biochem/physiol Actions

Increased concentrations of α-fetoprotein (AFP) in serum are less common in patients with malignancies of the gastrointestinal tract and of other organ systems with massive hepatic metastases. Hence, AFP determination is useful for the diagnosis of certain germ line tumors that contain yolk sac structures (testicular and ovarian tumors), hepatocellular carcinoma (hepatoma), and some malignant tumors of the gastrointestinal tract. AFP serves as an important marker for certain genetic and embryonic defects. Routine screening of pregnancies with a risk of neural tube defects (spina bifida, anencephaly, and Down′s syndrome) may be facilitated by the monitoring of AFP levels.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable