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Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat

Name: Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat
Brand: SIGMA
Price: 4479.5 CZK

affinity isolated antibody, buffered aqueous glycerol solution

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Pack Size	SKU	Availability	Price
0.25 mL		Please contact Customer Service for Availability	CZK 5,270.00 CZK 4,479.50

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162626

Conjugate:

alkaline phosphatase conjugate

Clone:

polyclonal

Application:

ELISA (d), IHC (p), WB CL

Citations:

CZK 4,479.50

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biological source

goat

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well)

shipped in

wet ice

storage temp.

2-8°C

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Show Differences

1 of 1

This Item	A1418	A3688	A9309
Sigma-Aldrich A7434 Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A1418 Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A3688 Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A9309 Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) direct ELISA: 1:40,000, western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20	technique(s) direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25, western blot: 1:50,000-1:100,000 using total cell extract of HeLa cells	technique(s) direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000	technique(s) direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:40,000-1:80,000 using total cell extract of HeLa cells
conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate peroxidase conjugate
biological source goat	biological source goat	biological source goat	biological source goat
form buffered aqueous glycerol solution	form buffered aqueous solution	form buffered aqueous glycerol solution	form buffered aqueous solution
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C

General description

Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.

Immunogen

Purified mouse IgG, Fc fragment

Application

Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37°C. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37°C.

Biochem/physiol Actions

Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human or rat samples.

Other Notes

Antibody adsorbed with human IgG and rat serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000470976

0000453311

0000416867

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Global Trade Item Number

SKU	GTIN
A7434-.25ML	04061837514593
A7434-1ML	04061835780358
A7434-.5ML	04061835805631

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