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A7189

L-Alanine Dehydrogenase from Bacillus subtilis

ammonium sulfate suspension, ≥20 units/mg protein (Lowry)

Synonym(s):

L-Alanine: NAD+ oxidoreductase (deaminating)

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250 units
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CZK 11,300.00
500 units
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CZK 21,700.00

About This Item

CAS Number:
9029-06-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-847-9
MDL number:
MFCD00131441
EC Number:
1.4.1.1 (BRENDA, IUBMB)
Specific activity:
≥20 units/mg protein (Lowry)
Biological source:
Bacillus subtilis

CZK 11,300.00

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biological source

Bacillus subtilis

Quality Segment

200

form

ammonium sulfate suspension

specific activity

≥20 units/mg protein (Lowry)

storage temp.

2-8°C

General description

L-Alanine Dehydrogenase has a N-terminal substrate-binding domain and a C-terminal NAD-binding domain.

Application

L-Alanine Dehydrogenase from Bacillus subtilis has been used in the carbon nanotube columns for H2-driven biocatalysis hydrogenation studies.
L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection [1].

Biochem/physiol Actions

L-Alanine Dehydrogenase is essential for sporulation in Bacillus subtilis.
L-Alanine dehydrogenase is a stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate and NADH are released in that specific order.[1] Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.[2]

Physical form

Suspension in 2.4 M (NH4)2SO4 solution, pH 7.0

Other Notes

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH3 per min at pH 10.0 at 25 °C.

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L-Alanine Dehydrogenase from Bacillus subtilis ammonium sulfate suspension, ≥20 units/mg protein (Lowry)

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Sigma-Aldrich

L2011

D-Lactic Dehydrogenase from Lactobacillus leichmannii

L-Leucine Dehydrogenase from Bacillus cereus lyophilized powder, ≥60 units/mg protein

Sigma-Aldrich

L5135

L-Leucine Dehydrogenase from Bacillus cereus

specific activity

≥20 units/mg protein (Lowry)

specific activity

~30 units/mg protein (Lowry)

specific activity

≥250 units/mg protein (biuret)

specific activity

≥60 units/mg protein

biological source

Bacillus subtilis

biological source

Bacillus subtilis

biological source

-

biological source

-

form

ammonium sulfate suspension

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

7783-20-2

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Certificates of Analysis (COA)

Lot/Batch Number
0000262370
0000274150
0000266670
0000274150
0000266670

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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Global Trade Item Number

SKUGTIN
A7189-250UN04061838168511
A7189-500UN04061838168528

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