SAE0023

Alanine Racemase from Bacillus stearothermophilus

buffered aqueous solution, ≥10 IU/mg solid

• Synonym(s): Alanine Racemase from Bacillus stearothermophilus
• CAS Number: 9024-06-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 5.1.1.1 (BRENDA, IUBMB)
• MDL number: MFCD03454300
• Specific activity: ≥10 IU/mg solid
• Biological source: Bacillus sp. (B. stearothermophilus)

Properties

• biological source: Bacillus sp. (B. stearothermophilus)
• Quality Segment: 200
• form: buffered aqueous solution
• specific activity: ≥10 IU/mg solid
• mol wt: M_r 78 kDa (2 subunits 39 kDa each)
• storage temp.: −20°C

Description

Application

Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-¹⁴C]alanine to a racemic mixture of L/D- [¹⁴C]alanine †.

Biochem/physiol Actions

Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†

Physical form

Aqueous solution containing 10 mM potassium phosphate

Other Notes

One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable