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A5222

Sigma-Aldrich

D-Amino Acid Oxidase from porcine kidney

powder, ≥1.5 units/mg solid

Synonym(s):

D-AAO, D-Amino acid: oxygen oxidoreductase (deaminating)

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100 UNITS
CZK 6,180.00
200 UNITS
CZK 10,500.00
500 UNITS
CZK 21,000.00

CZK 6,180.00


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100 UNITS
CZK 6,180.00
200 UNITS
CZK 10,500.00
500 UNITS
CZK 21,000.00

About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

CZK 6,180.00


Please contact Customer Service for Availability

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form

powder

specific activity

≥1.5 units/mg solid

storage temp.

−20°C

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Application

D-amino acid oxidase (DAO) is a potential target molecule for the treatment of chronic pain and schizophrenia [1][2]. D-amino acid oxidase is used to oxidize D-amino acids to their corresponding imino acids.

Unit Definition

One unit will oxidatively deaminate 1.0 μmole of D-alanine to pyruvate per minute at pH 8.3 at 25 °C, in the presence of a catalase.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Jin-Lu Huang et al.
Amino acids, 43(5), 1905-1918 (2012-09-22)
D-Amino acid oxidase (DAAO), a FAD-dependent peroxisomal flavoenzyme that catalyzes oxidation of D-amino acids to hydrogen peroxide, is distributed in the spinal cord almost exclusively expressed within astrocytes. The present study aims to explore potential contributions of spinal DAAO to
Norihiro Shibuya et al.
Nature communications, 4, 1366-1366 (2013-01-24)
In eukaryotes, hydrogen sulphide acts as a signalling molecule and cytoprotectant. Hydrogen sulphide is known to be produced from L-cysteine by cystathionine β-synthase, cystathionine γ-lyase and 3-mercaptopyruvate sulfurtransferase coupled with cysteine aminotransferase. Here we report an additional biosynthetic pathway for
Natsuki Matsumoto et al.
Methods in molecular biology (Clifton, N.J.), 1887, 23-31 (2018-12-07)
Fermented foods produced using edible microorganisms have been consumed worldwide since ancient times. Microorganisms produce some healthy functional substances. Recently, many people have been attracted to the notion that D-body amino acids have healthy functional effects. D-body amino acids convert
Andrea V Canosa et al.
Scientific reports, 8(1), 9016-9016 (2018-06-15)
Serine racemase (SR) catalyses two reactions: the reversible racemisation of L-serine and the irreversible dehydration of L- and D-serine to pyruvate and ammonia. SRs are evolutionarily related to serine dehydratases (SDH) and degradative threonine deaminases (TdcB). Most SRs and TdcBs
Cindy J Lee et al.
Metabolites, 12(9) (2022-09-24)
The pancreatic islets of Langerhans are clusters of cells that function as endocrine units synthesizing and releasing insulin and a range of additional peptide hormones. The structural and chemical characteristics of islets change during type 2 diabetes development. Although a

Questions

1–2 of 2 Questions  
  1. If I store it at -4 degrees Celsius instead of -20 degrees Celsius, will the enzyme denature so much that it cannot be used? Also, if I expose it to room temperature for use in an experiment and then store it at -4 degrees Celsius, will it be usable again

    1 answer
    1. This product should be stored at -20 °C as a powder. Information on storing the product in solution is not available, and it is recommended to prepare the product fresh before use.

      Helpful?

  2. Hello, I am doing my research about D-amino acid oxidase from porcine kidney. For further calculations I need molar extinction coefficient of pkDAAO. Maybe You have bigger description or some kind of information about it? Thank You.

    1 answer
    1. There are two extinction coefficient values available:

      Assuming all Cys residues form cystines - 1.935 at 280 nm in water, 0.1% (1 g/L)
      Assuming all Cys residues are reduced - 1.929 at 280 nm in water, 0.1% (1 g/L)

      Please see the link below to review this and additional information available at Expasy:
      https://web.expasy.org/cgi-bin/protparam/protparam1?P00371@noft@

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