This product is intended for research use only. Currently, a diagnostic or food grade product is not available. This product is not suitable for any other purpose, which includes, but is not limited to, unauthorized commercial use, in vitro diagnostic use, or therapeutic use in humans or animals.
G7141
Glucose Oxidase from Aspergillus niger
Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen)
Synonym(s):
β-D-Glucose:oxygen 1-oxidoreductase, G.Od., GOx
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| Size/SKU | Availability | Price |
|---|---|---|
10000 units | Please contact Customer Service for Availability | CZK 1,780.00 |
50000 units | Please contact Customer Service for Availability | CZK 3,330.00 |
250000 units | Please contact Customer Service for Availability | CZK 12,700.00 |
1000000 units | Please contact Customer Service for Availability | CZK 36,000.00 |
2500000 units | Please contact Customer Service for Availability | CZK 78,600.00 |
About This Item
CAS Number:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-601-0
MDL number:
Specific activity:
100,000-250,000 units/g solid (without added oxygen)
type
Type X-S
Quality Segment
form
lyophilized powder
specific activity
100,000-250,000 units/g solid (without added oxygen)
mol wt
160 kDa
composition
Protein, ≥65%
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
application(s)
diagnostic assay manufacturing
foreign activity
Catalase ≤5 units/mg protein
greener alternative category
storage temp.
−20°C
SMILES string
O1[C@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O)O)O)O
InChI
1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6-/m1/s1
InChI key
WQZGKKKJIJFFOK-VFUOTHLCSA-N
General description
Molecular Weight: 160 kDa (gel filtration)
pI: 4.2
Extinction coefficient: E1% = 16.7 (280 nm)
Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation.
Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates.
The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for β-D-glucose with a KM of 33-110 mM.
Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.
Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction.
pI: 4.2
Extinction coefficient: E1% = 16.7 (280 nm)
Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation.
Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates.
The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for β-D-glucose with a KM of 33-110 mM.
Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.
Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in fuel cell research. For more information see the article in biofiles.
Mass of glucose oxidase (GOX), a flavoprotein ranges from approximately 130 to 175 kDa. Some fungi and insects are capable of producing GOX.
Application
Glucose oxidase is widely used in the food and pharmaceutical industries as well as a major component of glucose biosensors.
Glucose Oxidase from Aspergillus niger has been used:
- in the (glucose oxidase) GO reagent to measure the glucose content by the glucose oxidase (GO) method
- to activate the human renal carcinoma cell line for constructing the oxidative stress model
- to study its influence in the paste on the analytical performance of the bioelectrode
Biochem/physiol Actions
Glucose oxidase catalyses the oxidation of β-d-glucose to d-glucono-β-lactone and hydrogen peroxide, with molecular oxygen as an electron acceptor.
Glucose oxidase (GOX) oxidizes D-glucose to D-gluconolactone and hydrogen peroxide. Hydrogen peroxide, the catalytic product of GOX, serves as an anti-bacterial and anti-fungal agent. It is safe and can be used in several industrial applications like baking, dry egg powder production, wine production, gluconic acid production, etc. It possesses electrochemical activity, which makes it extremely important in glucose sensors and in fuel cell applications.
Analysis Note
May contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase.
Protein determined by biuret
Other Notes
One unit will oxidize 1.0 μmole of β-D-glucose to D-gluconolactone and H2O2 per min at pH 5.1 at 35 °C, equivalent to an O2 uptake of 22.4 μl per min. If the reaction mixture is saturated with oxygen, the activity may increase by up to 100%.
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This Item | |||
|---|---|---|---|
| specific activity 100,000-250,000 units/g solid (without added oxygen) | specific activity ≥10,000 units/g solid (without added oxygen) | specific activity ≥100,000 units/g solid (without added oxygen) | specific activity ≥250 units/mg solid |
| form lyophilized powder | form powder | form lyophilized powder | form lyophilized solid |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing | application(s) - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt 160 kDa | mol wt 160 kDa | mol wt 160 kDa | mol wt - |
| foreign activity Catalase ≤5 units/mg protein | foreign activity amylase ≤0.5%, catalase ≤2 Sigma units/mg solid, galactose oxidase ≤3%, glycogenase ≤0.5%, invertase ≤0.5%, maltase ≤2% | foreign activity Catalase ≤10 Sigma units/mg protein | foreign activity Amylase ≤0.1%, catalase ≤20 U/mg (dry weight), sucrase ≤0.1% |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Is this GOx intended for diagnostic/biosensor use (not just food-industry)?
1 answer-
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Hi, I have two questions. 1,What is the total amount of this product. Actually ı need 27300 u, do you have it?.
1 answer-
For a 27,300 unit requirement, the 50,000 unit package size is recommended. The mass weight contained in the 50,000 unit package size may range from 50 – 500mg, based on the activity specification of 100,00 – 250,000 units per gram. Please see the link below to review a sample or lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/sigma/g7141#product-documentationHelpful?
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According to the size of the container that you describe for each product, how can I estimate its size in grams?
1 answer-
This product is sold by "units of activity". The activity specification for this item is 100,000-250,000 units/g solid. The exact result will be reported on the lot-specific Certificate of Analysis.
For example, lot SLCR6049 has a specific activity of 151,821 units/gram. Dividing the 10,000 unit pack size by the lot-specific activity will reveal the mass weight within the vial - the 10KU package size for this lot contains 65.8 µg.
Please see the link below to review a sample or lot specific Certificate:https://www.sigmaaldrich.com/product/sigma/g7141#product-documentation
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How should solutions of Glucose oxidase, Product G7141, be stored?
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No solution stability studies have been performed by Sigma. However, we would expect solutions prepared in buffer in the pH range of 5-7 to be stable at 2-8°C for at least one week. For longer term storage, solutions can be stored as frozen aliquots at -20°C (only one freeze/thaw cycle) for at least 6 months.
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What buffer is used during the preparation of Glucose oxidase, Product G7141?
1 answer-
Glucose oxidase contains potassium phosphate and tris acetate salts (which were present in the lyophilization buffer).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Glucose oxidase, Product G7141, inhibited by sodium azide, phenol, heparin, citrate or EDTA?
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Glucose oxidase is not inhibited by sodium azide (1.5 mM), low concentrations of phenol (10 mM), heparin, citrate or EDTA.
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