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A3653

Actin from bovine muscle

lyophilized powder

Synonym(s):

G-Actin

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About This Item

CAS Number:
51005-14-2
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD00130338
Form:
lyophilized powder
Assay:
≥90% (SDS-GE)
Biological source:
bovine muscle
Mol wt:
43 kDa

biological source

bovine muscle

Quality Level

200

assay

≥90% (SDS-GE)

form

lyophilized powder

mol wt

43 kDa

composition

Protein, ≥80% biuret

technique(s)

western blot: suitable

UniProt accession no.

P62739

storage temp.

2-8°C

Gene Information

bovine ... LOC(528168)

General description

Actin is a highly abundant and conserved protein. It can transition between the monomeric (G-actin) and filamentous (F-actin) forms, which is influenced by external factors. Actin is expressed in smooth, skeletal and cardiac muscles.

Application

Actin from bovine muscle has been used as an internal standard and positive control during western blotting.

Biochem/physiol Actions

Actin is involved in a wide variety of cellular processes like cell migration, phagocytosis, cell polarity and shape. It associates with myosin and aids in muscle contraction. The protein acts as a building block of microfilaments.

Physical form

Lyophilized powder containing Tris, ATP and CaCl2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLBX5919
SLCB8061
SLBQ7282V
SLBM5951V
SLBD7277V

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Articles

Actin Protein

Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.

Contrasting cellular stress responses of Baikalian and Palearctic amphipods upon exposure to humic substances: environmental implications
Protopopova MV, et al.
Environmental Science and Pollution Research International, 21(24), 14124-14137 (2014)
Laure Diancourt et al.
Toxins, 11(3) (2019-03-03)
Clostridiumperfringens type E is a less frequently isolated C.perfringens type and has not previously been reported in France. We have characterized two recent type E isolates, C.perfringens 508.17 from the intestinal content of a calf that died of enterotoxemia, and
Rheumatoid synovial fibroblasts constitutively express the fibrinolytic pattern of invasive tumor-like cells
Guiducci S, et al.
Clinical and Experimental Rheumatology, 23(3), 364-372 (2005)
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Global Trade Item Number

SKUGTIN
A3653-1MG04061826670880
A3653-5MG04061833267363

Questions

  1. Can you provide the protocol to polymerize the Actin from Bovine muscle?

    1 answer

    1. In the absence of salts, actin exists as a globular monomeric protein (G-actin). In the presence of ATP, a divalent cation (Ca2+ or Mg2+) and high salts (KCl, NaCl or MgCl2), the G-actin polymerizes to form a fibrous or filamentous actin (F-actin) which incorporates the cation and ATP (3,4). Dialysis of the F-actin solution (viscous) against water reverses the polymerization (2). The actin as it is supplied in product A3653 has been extensively dialyzed to be converted into G-Actin.

      This is described in the product information sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/313/659/a3653pis.pdf

      References
      2. Sigma quality control and production.
      3. Carlier, M.-F., J. Biol. Chem., 266, 1-4 (1991) - citing Kabsch, W. et al., Nature, 347, 37-44 (1990).
      4. Merck Index, 12th Ed., #137 (1996).

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