A2189

Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase, Mouse monoclonal

Name: Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase, Mouse monoclonal
Brand: SIGMA
Price: 6160 CZK

clone MB-11, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase

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0.2 ML

CZK 6,160.00

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About This Item

NACRES:

NA.77

UNSPSC Code:

12352203

Conjugate:

alkaline phosphatase conjugate

Clone:

MB-11, monoclonal

Application:

DB, ELISA (d)

Citations:

Key Documents

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biological source

mouse

Quality Level

300

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

MB-11, monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:35,000, dot blot: 1:20,000 using 20 ng of human IgM/dot, dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence)

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Related Categories

Antibodies - Primary, Secondary & Recombinant Monoclonal

Protein Biology

Secondary Antibodies

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1 of 4

This Item	A3437	A3076	A9794
Sigma-Aldrich A2189 Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase, Mouse monoclonal	Sigma-Aldrich A3437 Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A3076 Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal	Sigma-Aldrich A9794 Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in goat
antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody
conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate
species reactivity human	species reactivity human	species reactivity human	species reactivity -
technique(s) direct ELISA: 1:35,000, dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence), dot blot: 1:20,000 using 20 ng of human IgM/dot	technique(s) direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000	technique(s) direct ELISA: 1:6,000, dot blot: 1:1,000	technique(s) direct ELISA: 1:50,000, dot blot: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:60,000
biological source mouse	biological source goat	biological source mouse	biological source goat
isotype IgG2b	isotype -	isotype IgG2b	isotype -

General description

Human IgMs are the initial immunoglobulin isotypes that appear in blood in response to the first exposure to external antigens. IgMs have been implicated in CNS myelin repair and increased secretory IgM levels have been linked to rhino-conjunctivitis . Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM. The product does not react with human IgG, IgA or light chains.

Monoclonal Anti-Human IgM (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

Human IgM isolated from pooled normal human serum

Application

Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody purified from hybridoma cell culture has been used in immunocytochemistry and enzyme linked immunosorbent assay (ELISA)

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Analysis of epitope-specific immune responses induced by vaccination with structurally folded and unfolded recombinant Bet v 1 allergen derivatives in man.

Ines Pree et al.

Journal of immunology (Baltimore, Md. : 1950), 179(8), 5309-5316 (2007-10-04)

Previously, we have constructed recombinant derivatives of the major birch pollen allergen, Bet v 1, with a more than 100-fold reduced ability to induce IgE-mediated allergic reactions. These derivatives differed from each other because the two recombinant Bet v 1

A natural human IgM that binds to gangliosides is therapeutic in murine models of amyotrophic lateral sclerosis

Xu X, et al.

Disease models & mechanisms, 8(8), 831-842 (2015)

Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen.

Ana Luísa Tomás et al.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 39(11), 2205-2209 (2020-06-20)

Interest in the detection of specific anti-Pneumocystis jirovecii antibodies has emerged as less-invasive alternative diagnostic approaches. Here is presented the performance of an ELISA based on a recombinant synthetic multi-epitope kexin 1 (Kex1) antigen of P. jirovecii, previously developed. Results

Human monoclonal IgM antibody promotes CNS myelin repair independent of Fc function.

Bogoljub Ciric et al.

Brain pathology (Zurich, Switzerland), 13(4), 608-616 (2003-12-06)

The human monoclonal IgM antibody sHIgM22 and mouse IgM monoclonal antibody 94.03 bind to oligodendrocytes, induce calcium signals in cultured glial cells, and promote remyelination in mouse models of multiple sclerosis. In order to address the mechanisms employed by these

Detection of secretory IgM in tears during rhino-conjunctivitis.

Johan Bours et al.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 243(5), 456-463 (2005-06-03)

With various methods, secretory immunoglobulin M (sIgM) was assessed in tears of patients with rhino-conjunctivitis. Tears were analyzed by microimmunoelectrophoresis (MIE), size-exclusion high-pressure liquid chromatography (SE-HPLC), sodium dodecyl sulphate (SDS) electrophoresis and isoelectric focusing. Only very small traces of serum

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Global Trade Item Number

SKU	GTIN
A2189-.2ML	04061838352149

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