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73737

4-Nitrophenyl phosphate bis(tris) salt

phosphatase substrate, ≥97.0% (enzymatic), powder or crystals

Synonym(s):

p-Nitrophenylphosphate bis(tris) salt, p-nitrophenyl phosphate bis(tris) salt, PNPP, PNPP bis(tris) salt

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5 g
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CZK 4,500.00
25 g
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CZK 16,900.00
CZK 14,365.00

About This Item

Linear Formula:
(C6 H4 N O6 P)( C4 H12 N O3)2
CAS Number:
68189-42-4
Molecular Weight:
461.36
NACRES:
NA.32
PubChem Substance ID:
57652488
UNSPSC Code:
12352204
EC Number:
269-198-6
MDL number:
MFCD00037009
Beilstein/REAXYS Number:
1578142

CZK 4,500.00

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Product Name

4-Nitrophenyl phosphate bis(tris) salt, for the determination of phosphatase, ≥97.0% (enzymatic)

Quality Segment

100

assay

≥97.0% (enzymatic)

form

powder or crystals

quality

for the determination of phosphatase

impurities

≤0.1% free nitrophenol, ≤6% water

solubility

H2O: 0.1 g/mL, clear

storage temp.

2-8°C

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

Application

4-Nitrophenyl phosphate bis(tris) salt has been used to determine the enzyme activity of acid and alkaline phosphatase in cell lysates.[1]

Biochem/physiol Actions

4-Nitrophenyl phosphate is a non-proteinaceous and non-specific chromogenic substrate,[2] preferred for alkaline phosphatase (ALP) in enzyme-linked immunosorbent assay (ELISA). It is widely used to assay protein tyrosine phosphatases (PTPs).[2] Upon cleavage, the substrate produces a yellow product that is spectrophotometrically measured.

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This Item
N3254N3129N3002
4-Nitrophenyl phosphate bis(tris) salt for the determination of phosphatase, ≥97.0% (enzymatic)

Sigma-Aldrich

73737

4-Nitrophenyl phosphate bis(tris) salt

4-Nitrophenyl phosphate di(tris) salt phosphatase substrate

Sigma-Aldrich

N3254

4-Nitrophenyl phosphate di(tris) salt

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt phosphatase substrate

Sigma-Aldrich

N3129

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

Bis(p-nitrophenyl) phosphate sodium salt ≥99% (TLC)

Sigma-Aldrich

N3002

Bis(p-nitrophenyl) phosphate sodium salt

assay

≥97.0% (enzymatic)

assay

≥97% (enzymatic)

assay

≥97% anhydrous basis

assay

≥99% (TLC)

solubility

H2O: 0.1 g/mL, clear

solubility

water: 50 mg/mL, clear, colorless to light yellow

solubility

water: 10 mg/mL, clear, colorless to faintly yellow

solubility

water: 20 mg/mL, clear to very slightly hazy

Quality Level

100

Quality Level

100

Quality Level

300

Quality Level

200

form

powder or crystals

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

quality

for the determination of phosphatase

quality

-

quality

-

quality

-

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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