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49429

Phalloidin–Atto 532

suitable for fluorescence, ≥90.0% (HPLC)

Synonym(s):

Atto 532–Phalloidin

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10 nmol
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CZK 15,200.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352108

CZK 15,200.00

Please contact Customer Service for Availability
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Quality Level

100

assay

≥90.0% (HPLC)

manufacturer/tradename

ATTO-TEC GmbH

λ

in acetonitrile: water (1:9) (with 0.01% trifluoroacetic acid)

UV absorption

λ: 534-540 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 532 is a novel fluorescent label related to the well-known dye Rhodamine 6G. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, excellent water solubility, and very little triplet formation. After coupling to a substrate Atto 532 Phalloidin carries a net electrical charge of -1. Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10–8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours.

Application

Fluorescent conjugates of phalloidin, rhodamine-phalloidin staining reagents, such as Phalloidin–Atto 532 are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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519271884666939
Sigma-Aldrich

Sigma-Aldrich

49429

Phalloidin–Atto 532

Sigma-Aldrich

Sigma-Aldrich

51927

Phalloidin–Atto 594

Sigma-Aldrich

Sigma-Aldrich

18846

Phalloidin–Atto 655

Sigma-Aldrich

Sigma-Aldrich

66939

Phalloidin–Atto 425

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

assay

≥90.0% (HPLC)

assay

≥90.0% (HPLC)

assay

≥90.0% (sum of isomers, HPLC)

assay

≥90% (HPLC)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

UV absorption

λ: 534-540 nm Amax

UV absorption

λ: 600-606 nm Amax

UV absorption

λ: 651- 657 nm Amax

UV absorption

λ: 429-435 nm Amax

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL7603
BCCL5711
BCCH4070
BCCH2994
BCCD4326

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Articles

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Models of the collective behavior of proteins in cells: tubulin, actin and motor proteins.
Tuszynski JA, Brown JA, Sept D.
J. Biol. Physics, 29, 401-428 (2003)
J E Bowe et al.
Diabetologia, 56(4), 783-791 (2013-01-25)
Glucose plays two distinct roles in regulating insulin secretion from beta cells--an initiatory role, and a permissive role enabling receptor-operated secretagogues to potentiate glucose-induced insulin secretion. The molecular mechanisms underlying the permissive effects of glucose on receptor-operated insulin secretion remain
Magdalena Izdebska et al.
Acta histochemica, 115(5), 487-495 (2013-01-15)
Quantum dots (QDs) are fluorescent nanocrystals whose unique properties are fundamentally different from organic fluorophores. Moreover, their cores display sufficient electron density to be visible under transmission electron microscopy (TEM). Here, we report a technique for phalloidin-based TEM detection of
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Global Trade Item Number

SKUGTIN
49429-10NMOL04061832532233

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