479M-9

MDM2 (IF2) Mouse Monoclonal Antibody

• Synonym(s): Mouse double minute protein 2
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: IF2, monoclonal
• Application: IHC (p)
• Citations: 8

Properties

• biological source: mouse
• Quality Segment: 100, 500
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: IF2, monoclonal
• description: For In Vitro Diagnostic Use in Select Regions
• form: buffered aqueous solution
• species reactivity: human
• packaging: vial of 0.1 mL concentrate (479M-94), vial of 0.1 mL concentrate Research Use Only (479M-94-RUO), vial of 0.5 mL concentrate (479M-95), vial of 1.0 mL concentrate (479M-96), vial of 1.0 mL concentrate Research Use Only (479M-96-RUO), vial of 1.0 mL pre-dilute Research Use Only (479M-97-RUO), vial of 1.0 mL pre-dilute ready-to-use (479M-97), vial of 7.0 mL pre-dilute ready-to-use (479M-98), vial of 7.0 mL pre-dilute ready-to-use Research Use Only (479M-98-RUO)
• manufacturer/tradename: Cell Marque^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:50 (concentrated)
• isotype: IgG
• control: dedifferentiated liposarcoma, well-differentiated liposarcoma
• shipped in: wet ice
• storage temp.: 2-8°C
• visualization: nuclear

Description

General description

Mouse double minute protein 2 (MDM2) is a gene encoded on the 12q13-14 chromosomal sequence.1-5 It encodes for a 483 amino acid residue protein which binds to the amino-terminal transcription region of p53.^2,5 MDM2 has been shown to negatively regulate the tumor-suppressor activity of p53 by three mechanisms: Blocking p53 transcription, binding to p53 causing it to be exported from the nucleus, and accelerating the destruction of p53.¹ MDM2 up-regulation has been shown in liposarcoma while being absent in lipoma.^2,4 Therefore, anti-MDM2 has been demonstrated to be a potentially useful tool in distinguishing well-differentiated liposarcoma (atypical lipomatous tumor) from lipoma, with the neoplastic cells positive in the former lesion and negative in lipoma.^2,4

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide.

Preparation Note

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Analysis Note

United States - IVD
Canada - RUO
European Union - IVD
Japan - RUO

Other Notes

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Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany