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41658

Lipase A Candida antarctica immobilized on Immobead 150, recombinant from Aspergillus oryzae

≥500 U/g

Synonym(s):

Candida antarctica Lipase

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Size/SKUAvailabilityPrice
10 g
Please contact Customer Service for Availability
CZK 3,960.00
50 g
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CZK 15,200.00
CZK 12,920.00

About This Item

NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥500 U/g
Recombinant:
expressed in Aspergillus oryzae

CZK 3,960.00


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recombinant

expressed in Aspergillus oryzae

Quality Segment

form

beads (powder like), beads

specific activity

≥500 U/g

storage temp.

2-8°C

General description

Research area: Cellsignalling. Lipase A Candida Antarctica, CalA is a thermostable, calcium-dependent enzyme with high substrate specificity. CalA comprises of the catalytic triad (Ser184, Asp334, His366) and has an α/β hydrolase structural fold.

Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Lipase A Candida antarctica immobilized on Immobead 150, recombinant from Aspergillus oryzae has been used in the synthesis of enantiopure (R)-salsolinol and adsorption kinetics studies using Quartz crystal microbalance with dissipation (QCM-D).It has also been used tostudy the esterification of difluorinated alcohols.[1]

Biochem/physiol Actions

Lipase A Candida Antarctica, CalA is highly specific for alcohols and esterifies the trans-isomer of fatty acids. CalA recognizes highly branched acyl groups and is active on alcohols with steric hindrance. CalA catalyzes the production of enantiopure amino acids and aids in the synthesis of chiral cyanohydrins. It may find industrial applications for its thermostable functionality in paper industry.Lipase A Candida antarctica(CAL-A) shows acetyltransferase activity by synthesizing fatty acid esters from certain alcohols and natural oils in an aqueous environment.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 10.0 and 40°C (tributyrin, Cat. No. 91010, as substrate)

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543265258362288
specific activity

≥500 U/g

specific activity

≥1800 U/g

specific activity

≥2000 U/g

specific activity

~9 U/mg

recombinant

expressed in Aspergillus oryzae

recombinant

expressed in Aspergillus oryzae

recombinant

expressed in yeast

recombinant

expressed in Aspergillus oryzae

form

beads (powder like)

form

beads

form

beads

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

  1. What is the solvent of Lipase A Candida antarctica immobilized on Immobead 150, recombinant from Aspergillus oryzae? What is it soluble in?

    1 answer
    1. This product is Lipase A from Candida antarctica that is covalently attached to an apolar macroporous carrier. This product is not directly screened for solubility. This item is commonly used in aqueous hydrolysis activity assays using 1-10% tributyrin in 25 mM potassium phosphate buffer, pH 7.5 at 40 °C.

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