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32855

Eosin Methylene blue

according to May-Grünwald

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About This Item

UNSPSC Code:
12171500
NACRES:
MA.02
PubChem Substance ID:
329754361
MDL number:
MFCD00005038
Colour Index Number:
45380/52015

form

powder or crystals

quality

according to May-Grünwald

εmax

≥1000 at 635-655 nm in methanol, ≥650 at 512-532 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

InChI

1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1

InChI key

NXBZXYJXNIFCRJ-UHFFFAOYSA-M

General description

Eosin has been used in combination with methylene blue, to detect carboxlic acid group in organic compounds. It gives a red or orange-coloured precipitate on treatment with carboxylic acid solution in water, acetone, alcohol, or dioxin. It is also used as an indicator for differentiation of the colon from the typhoid-dysentery group of bacteria.
Mixture of eosin and methylene blue stains for use in differential microbiological media to culture and identify pathogenic microorganisms, e.g., Neisseria sicca,, Acinetobacter calcoaceticus.

Application

Eosin methylene blue was used for staining cells, during optical microscopy studies, done for analysis of cell morphology.

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230251M914095290
Eosin Methylene blue according to May-Grünwald

Sigma-Aldrich

32855

Eosin Methylene blue

Eosin Y 75% (HPLC)

Sigma-Aldrich

230251

Eosin Y

Methylene blue certified by the BSC

Sigma-Aldrich

M9140

Methylene blue

Methyl Blue suitable for microscopy

Sigma-Aldrich

95290

Methyl Blue

form

powder or crystals

form

powder

form

powder

form

powder or crystals

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

quality

according to May-Grünwald

quality

-

quality

-

quality

-

εmax

≥1000 at 635-655 nm in methanol

εmax

-

εmax

-

εmax

≥200 at 310-320 nm, ≥400 at 595-605 nm

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317,H319

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCBZ7880
BCBW4397
BCBS2100V
BCBS2100
BCBQ8960

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Detection of carboxylic acid group in organic compounds with eosin, mercurochrome, and methylene blue.
Johar GS.
Fresenius' Zeitschrift fur Analytische Chemie, 260 (2), 122-123 (1972)
The effect of concentration of dyes on differentiation of enteric bacteria on eosin-methylene-blue agar.
Journal of Bacteriology, 45 (5), 471-471 (1943)
Oncosis is associated with exposure of phosphatidylserine residues on the outside layer of the plasma membrane: a reconsideration of the specificity of the annexin V/propidium iodide assay.
Lecoeur H
Cytometry, 44 (1), 65-72 (2001)

Global Trade Item Number

SKUGTIN
32855-25G04061826717585

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