30970

Sodium deoxycholate

BioXtra, ≥98.0% (dry matter, NT)

• Synonym(s): 3α,12α-Dihydroxy-5β-cholanic acid sodium salt, 7-Deoxycholic acid sodium salt, Desoxycholic acid sodium salt
• Empirical Formula (Hill Notation): C₂₄H₃₉NaO₄
• CAS Number: 302-95-4
• Molecular Weight: 414.55
• Beilstein: 3581950
• EC Number: 206-132-7
• MDL number: MFCD00064139
• UNSPSC Code: 12161900
• PubChem Substance ID: 57648497
• NACRES: NA.21

Properties

• biological source: ox bile
• Quality Level: 300
• description: anionic
• product line: BioXtra
• Assay: ≥98.0% (dry matter, NT)
• form: powder
• optical activity: [α]20/D +44±2°, c = 2% in H₂O
• mol wt: micellar avg mol wt 1200-5000
• aggregation number: 3-12
• technique(s): DNA purification: suitable; RNA purification: suitable; electrophoresis: suitable
• impurities: ≤1% cholic acid (HPLC); ≤6% water
• CMC: 2-6 mM (20-25°C)
• solubility: water: soluble 1 gm in 10 ml, clear, colorless to very faintly yellow
• cation traces: Ca: ≤500 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤10 mg/kg; Mg: ≤10 mg/kg; Mn: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤10 mg/kg; Zn: ≤5 mg/kg
• HLB: 16
• functional group: carboxylic acid
• SMILES string: [Na+].[H][C@]12CC[C@@]3([H])[C@]4([H])CC[C@H]([C@H](C)CCC([O-])=O)[C@@]4(C)[C@@H](O)C[C@]3([H])[C@@]1(C)CC[C@@H](O)C2
• InChI: 1S/C24H40O4.Na/c1-14(4-9-22(27)28)18-7-8-19-17-6-5-15-12-16(25)10-11-23(15,2)20(17)13-21(26)24(18,19)3;/h14-21,25-26H,4-13H2,1-3H3,(H,27,28);/q;+1/p-1/t14-,15-,16-,17+,18-,19+,20+,21+,23+,24-;/m1./s1
• InChI key: FHHPUSMSKHSNKW-SMOYURAASA-M

Description

General description

Sodium Deoxycholate, a bile acid derivative, is a crucial component in biochemical and cell biology research. This water-soluble detergent is widely used in protein-related experiments, including cell lysates and liposome preparation. It aids in membrane protein extraction, lipid compound extraction, and cell nucleus separation. Sodium Deoxycholate is also applied in cholesterol flocculation tests and affinity chromatography for column elution or regeneration. It is commonly used in microbiology for cell lysing, especially because it can be removed from the solution via dialysis.

Sodium Deoxycholate is also utilized in eluting buffers for affinity chromatography and plays a crucial role in DNA and RNA extraction from cells and tissues in molecular biology applications. Additionally, it is used as a catalyst in the preparation of protein extracts for immunoblotting and immunoprecipitation of radiolabeled proteins.

Application

Sodium deoxycholate has been used for preparation of protein extracts for immunoblotting[1] and immunoprecipitation of radiolabeled proteins.[2]

Biochem/physiol Actions

Sodium deoxycholate is a bile salt that stimulates the apical to basolateral accumulation of epirubicin in Caco-2 cells[3] as well as increased serosal absorption of epirubicin in the rat jejunum and ileum.[4] Sodium deoxycholate dissociates histones II from DNA as well as causes lysis of the viral membrane.[5]

Features and Benefits

• Suitable for Cell Biology and Biochemical Research
• Can be used in Electrophoresis, DNA and RNA purification
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
• High purity product for research applications

Other Notes

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Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302
• Precautionary Statements: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves