294R-1

BOB.1 (SP92) Rabbit Monoclonal Antibody

• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: SP92, monoclonal
• Application: IHC (p)
• Citations: 14

Properties

• biological source: rabbit
• Quality Level: 100, 500
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: SP92, monoclonal
• description: For In Vitro Diagnostic Use in Select Regions (See Chart)
• form: buffered aqueous solution
• species reactivity: human
• packaging: vial of 0.1 mL concentrate (294R-14), vial of 0.5 mL concentrate (294R-15), bottle of 1.0 mL predilute (294R-17), vial of 1.0 mL concentrate (294R-16), bottle of 7.0 mL predilute (294R-18)
• manufacturer/tradename: Cell Marque^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
• isotype: IgG1
• control: B-cell lymphoma, tonsil
• shipped in: wet ice
• storage temp.: 2-8°C
• visualization: nuclear
• Gene Information: human ... POU2AF1(5450)

Description

General description

Expression of BOB.1/OBF.1 is restricted largely to mature B-cells. Germinal center B-cells normally demonstrate strong staining for BOB.1, as do mantle-zone B-cells, and plasma cells.[1] Analyses of BOB.1/OBF.1 expression in a variety of established B-cell lines representing different stages of B-cell development has suggested a constitutive, B-cell-specific expression pattern. Because they are germinal center derived, L&H cells in nodular lymphocyte predominant Hodgkin lymphoma are consistenty immunoreactive for BOB.1. Conversely, the Hodgkin/Reed- Sternberg cells in classical Hodgkin lymphoma either do not express both (80%) or express only one (20%) of the two proteins.[1][2][3][4][5] In B-cell lymphomas, the highest expression levels for BOB.1/OBF.1 are reported in follicular center lymphomas[6][7], diffuse large B-cell lymphomas[8][9] and Burkitt lymphomas.[9]B-CLL, MALT-type, and mantle cell lymphomas score negative or display an heterogenous/weaker reactivity.10-11 Studies have suggested that anti-BOB. has a high predictive value in discriminating primary mediastinal B-cell lymphoma from classical Hodgkin disease.[10][11]Approximately 50% of acute myeloid leukemias express BOB.1.[12]

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

BOB.1 Positive Control Slides, Product No. 294S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

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Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany