Compare the use of Proteinase K for 5 and 10 minutes, which should be sufficient. There's no need to run HIER or antigen retrieval, as the Enzyme should effectively open the epitope sites for this antibody.
248M-9
Ep-CAM/Epithelial Specific Antigen (Ber-EP4) Mouse Monoclonal Antibody
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
Ber-EP4, monoclonal
Application:
IHC (p)
Citations:
7
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biological source
mouse
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
Ber-EP4, monoclonal
description
For In Vitro Diagnostic Use in Select Regions (See Chart)
form
buffered aqueous solution
species reactivity
human
packaging
vial of 0.1 mL concentrate (248M-94), vial of 0.5 mL concentrate (248M-95), bottle of 1.0 mL predilute (248M-97), vial of 1.0 mL concentrate (248M-96), bottle of 7.0 mL predilute (248M-98)
manufacturer/tradename
Cell Marque®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200
isotype
IgG1κ
control
colon carcinoma
shipped in
wet ice
storage temp.
2-8°C
visualization
cytoplasmic
Gene Information
human ... EPCAM(4072)
General description
Epithelial cell adhesion molecule (Ep-CAM) is a transmembrane glycoprotein localized on the membrane of cells in most epithelial tissues. Immunoreactivity with the antibody to E-CAM has been seen in the majority of epithelial neoplasms, whereas most non-epithelial neoplasms do not show Ep-CAM expression.[1] Ep-CAM is not expressed in mesothelial cells, hepatocytes, and lymphocytes.[1] In conjunction with other markers, Ep-CAM can be used as an aid in determining the epithelial origin of neoplasms such as lung adenocarcinoma.[1] [2]
Physical form
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Preparation Note
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Analysis Note
 IVD |  IVD |  IVD |  RUO |
Other Notes
Ep-CAM Positive Control Slides, Product No. 248S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
For Technical Service please contact: 800-665-7284 or email: [email protected]
Legal Information
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
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Přehled diagnostické imunohistochemie zdůrazňuje její význam v diagnostice nádorových onemocnění a detekci infekčních chorob v moderní klinické patologii.
Diagnostic immunohistochemistry overview highlights its importance in cancer diagnosis and detecting infectious diseases in modern clinical pathology.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 248M-95 | 0841683130239 |
| 248M-97 | 0841683130253 |
| 248M-94 | 0841683130222 |
| 248M-98 | 0841683130260 |
| 248M-96 | 0841683130246 |
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how to use Proteinase K for the Ber-ep4?
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