One such filter set is the Zeiss Filter Set 01 (ex=359 nm, em=461 nm).
D9564
DAPI, dilactate
≥98% purity (HPLC), powder
DAPI, dilactate
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
4′,6-Diamidino-2-phenyindole, dilactate
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 mg | Please contact Customer Service for Availability | CZK 6,750.00 |
About This Item
Empirical Formula (Hill Notation):
C16H15N5 · 2C3H6O3
Molecular Weight:
457.48
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Product Name
DAPI, dilactate, ≥98% (HPLC)
Quality Level
assay
≥98% (HPLC)
form
powder
technique(s)
titration: suitable
color
yellow
solubility
H2O: 20 mg/mL
ε (extinction coefficient)
28.00-32.50 at 345-351 nm in methanol
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)
InChI key
LCAUPANIXBMNIA-UHFFFAOYSA-N
General description
DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.
Application
DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.
Biochem/physiol Actions
A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.
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This Item | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) transfection: suitable | technique(s) - | technique(s) - |
| color yellow | color - | color yellow | color - |
| form powder | form powder | form solid | form powder |
| solubility H2O: 20 mg/mL | solubility H2O: 20 mg/mL, PBS: insoluble | solubility water: 5 mg/mL, ethanol: soluble, methanol: soluble | solubility - |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) - |
| Quality Level 300 | Quality Level 300 | Quality Level 100 | Quality Level - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| D9564-10MG | 04061833590867 |
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Which filter sets can be used to visualize the fluorophore produced by Product D9564, DAPI, dilactate (DAPI-DNA)?
1 answer-
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Can Product D9564, DAPI dilactate (DAPI-DNA), be used as a vital stain?
1 answer-
As we note in the Product Information Sheet for the Dihydrochloride salt of DAPI, Product No. D9542, DAPI can be used as a vital dye to stain mature pollen grains, but we have tested it as a vital stain in other cell types.
Helpful?
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Isn't the hydrochloride salt form of DAPI a more sensitive detector of DNA than Product D9564, DAPI, dilactate (DAPI-DNA)?
1 answer-
The dilactate salt has as much sensitivity in detection as the dihydrochloride salt, which we also offer as Product No. D9542.
Helpful?
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