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A7409

Bovine Serum Albumin solution

35% in 0.85% sodium chloride, aseptically filled

Synonym(s):

Albumin solution from bovine serum, BSA solution

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Size/SKUAvailabilityPrice
10 mL
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CZK 2,870.00
50 mL
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CZK 8,920.00
1 L
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CZK 60,900.00

About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Form:
solution
Technique(s):
microbiological culture: suitable
Concentration:
33.0-37.4% protein (biuret), 35% in 0.85% sodium chloride

CZK 2,870.00


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biological source

bovine

Quality Segment

description

contains no preservative

sterility

aseptically filled

form

solution

purified by

heat shock fractionation

origin

USA origin

concentration

33.0-37.4% protein (biuret), 35% in 0.85% sodium chloride

technique(s)

microbiological culture: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

General description

Albumin belongs to the four-member albumin superfamily.[1] It is a major protein in the circulating blood.[2] Albumin has a helical structure with three domains that form a heart-like shape.[1]

Application

Bovine Serum Albumin solution has been used:
  • to block protein-coated well plates in enzyme-linked immunosorbent assay (ELISA) for immunoreactivity and cross-reactivity studies in the production of monoclonal antibodies (mAbs)[3]
  • to prepare primary antibody solution for peroxidases staining[4]
  • as a supplement for in vitro cultivation of Borrelia strains[4]

Biochem/physiol Actions

Bovine serum albumin (BSA) serves as a protein supplement for cell culture media. BSA acts as a transport carrier for drugs, and it is commonly used to study the protein-drug interactions. Furthermore, it is known for its ability to interact with several aromatic and hydrophobic molecules of the bloodstream.

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This Item
A7888A3912A8022
biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

technique(s)

microbiological culture: suitable

technique(s)

blood typing: suitable

technique(s)

blood typing: suitable, microbiological culture: suitable

technique(s)

ELISA: suitable, blocking: suitable, electrophoresis: suitable, western blot: suitable

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

300

form

solution

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

Gene Information

bovine ... ALB(280717)

Gene Information

bovine ... ALB(280717)

Gene Information

bovine ... ALB(280717)

Gene Information

bovine ... ALB(280717)


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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Questions

  1. Is the BSA cat. A7409 suitable for MACS buffer used for T cell separation? Separated cells would be cultured for around 2 weeks

    1 answer
    1. This product has not been tested for suitability in this application. While it is aseptically prepared, this product is not tested for sterility.

      See product A7979 for the sterile, cell culture tested BSA solution that may be more suitable: https://www.sigmaaldrich.com/product/sigma/a7979

      In addition, see product A9418 to review another cell culture tested product that is cited for use in MACS buffer: https://www.sigmaaldrich.com/product/sigma/a9418

      A link to this publication is below:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059065/

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