69899

Monochlorobimane

suitable for fluorescence, ≥70.0% (HPCE)

• Synonym(s): mBCl, Chlorobimane
• Empirical Formula (Hill Notation): C₁₀H₁₁ClN₂O₂
• CAS Number: 76421-73-3
• Molecular Weight: 226.66
• UNSPSC Code: 12352108
• NACRES: NA.32
• PubChem Substance ID: 329762099
• MDL number: MFCD00077379
• Beilstein/REAXYS Number: 4440901
• Assay: ≥70.0% (HPCE)
• Form: powder

Properties

• Quality Segment: 100
• assay: ≥70.0% (HPCE)
• form: powder
• mp: 135-136 °C (lit.)
• solubility: DMF: soluble, DMSO: soluble, acetonitrile: soluble, methanol: soluble
• fluorescence: λ_ex 380 nm; λ_em 461 nm in methanol, λ_ex 390 nm; λ_em 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione)
• suitability: suitable for fluorescence
• SMILES string: CC1=C(C)C(=O)N2N1C(CCl)=C(C)C2=O
• InChI: 1S/C10H11ClN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
• InChI key: SUIPVTCEECPFIB-UHFFFAOYSA-N

Description

General description

Monochlorobimane is a glutathione (GSH) fluorescent cell-permeable probe. When incubated with the test cell culture, it readily enters the cells and forms a fluorescent complex. The Monochlorobimane-GSH reaction is catalyzed by glutathione-S-transferase, which is detected fluorometrically.

Monochlorobimane, also known as mBCl, is a non-fluorescent compound that forms a fluorescent complex upon reaction. The fluorescence is detected at 394/490nm.

Application

Monochlorobimane is used as a fluorescent agent in fluorometric glutathione assays. It is used to detect the principal intracellular low-molecular-weight thiols, which play a pivotal role in the defense mechanism.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves