67309

Kovac′s reagent for indoles

suitable for microbiology

• Synonym(s): 4-(Dimethylamino)benzaldehyde solution
• Empirical Formula (Hill Notation): C₉H₁₁NO
• CAS Number: 100-10-7
• Molecular Weight: 149.19
• UNSPSC Code: 41171621
• NACRES: NA.85
• PubChem Substance ID: 329760541
• MDL number: MFCD00003381
• Beilstein/REAXYS Number: 4132845

Properties

• agency: according to ISO 16654:2001
• Quality Segment: 200
• product line: BioChemika
• shelf life: limited shelf life, expiry date on the label
• composition: 4-(dimethlyamino)benzaldehyde, 50 g/L , hydrochloric acid, 240 g/L , isoamylic alcohol, 710 g/L
• technique(s): microbe id | specific enzyme detection: suitable
• application(s): agriculture; clinical testing; environmental; food and beverages, microbiology
• storage temp.: 2-8°C
• suitability: Escherichia coli, coliforms
• SMILES string: [H]C(=O)c1ccc(cc1)N(C)C
• InChI: 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
• InChI key: BGNGWHSBYQYVRX-UHFFFAOYSA-N

Description

General description

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Application

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.

Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent.[1] It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.[2]

Safety Information

• pictograms: GHS02,GHS05,GHS07
• signalword: Danger
• hcodes: H226,H290,H314,H317,H332,H335
• pcodes: P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 3 - Flammable liquids
• wgk: WGK 3
• flash_point_f: 109.4 °F
• flash_point_c: 43 °C
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter