This has a maximum emission at 609 nm, and excitation 590nm
SCT021
BioTracker 609 Red Ca2+ AM Dye
Live cell imaging calcium (Ca2+) indicator for neuroscience and cancer applications.
Synonym(s):
Live cell calcium detection probe, Live cell imaging probe, Red fluorescent calcium indicator
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| Size/SKU | Availability | Price |
|---|---|---|
5 x 40 nmol | Available to ship TODAYfromAreál Kühne+Nagel spol. s r.o. | 16 300,00 CZK |
About This Item
UNSPSC Code:
12352207
NACRES:
NA.47
eCl@ss:
32161000
16 300,00 CZK
Available to ship TODAYDetails
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
storage temp.
-10 to -25°C
General description
Calcium (Ca2+) play a vital role in the physiology and biochemistry of the cell. Calcium ions play an important role in signal transduction pathways, neurotransmitter release from neurons, contraction of all muscle cell types, and in fertilization. Extracellular calcium is also important for maintaining the potential difference across excitable cell membranes, as well as proper bone formation.
The BioTracker 609 Red Ca2+ AM Dye is a live cell red fluorescent calcium indicator. Fluorescence intensity of this fluorescent probe reversibly changes depending on Ca2+ concentration. It has red fluorescence with maximum emission at 609 nm (Excitation 590nm) and is suitable for detection of micromolar- to submicromolar-range calcium ions. The dye is hydrolyzed by intracellular esterases to become soluble which equally distributes in the cytoplasm. This feature enables detection of intracellular calcium concentration changes with superior accuracy compared to other calcium indicators.
Spectral Properties
Absorbance: 595nm
Emission: 609nm
The BioTracker 609 Red Ca2+ AM Dye is a live cell red fluorescent calcium indicator. Fluorescence intensity of this fluorescent probe reversibly changes depending on Ca2+ concentration. It has red fluorescence with maximum emission at 609 nm (Excitation 590nm) and is suitable for detection of micromolar- to submicromolar-range calcium ions. The dye is hydrolyzed by intracellular esterases to become soluble which equally distributes in the cytoplasm. This feature enables detection of intracellular calcium concentration changes with superior accuracy compared to other calcium indicators.
Spectral Properties
Absorbance: 595nm
Emission: 609nm
Preparation Note
Store BioTracker 609 Red Ca2+ AM Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
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This Item | |||
|---|---|---|---|
| description Live cell imaging calcium (Ca2+) indicator for neuroscience and cancer applications. | description Live cell imaging red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342. | description Live cell imaging mitochondrial dye that stains the membrane of mitochondria used to detect cell viability, metabolic activity and overall cell health. | description Live cell imaging dye for glutathione (GSH) that detects reactive oxygen species (ROS) free radicals. |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| storage temp. -10 to -25°C | storage temp. 2-8°C | storage temp. -10 to -25°C | storage temp. -10 to -25°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
1202.4 °F
flash_point_c
650.2 °C
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please provide the excitation and emission spectra of BioTracker 609 Ca dye
1 answer-
Helpful?
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HOW LONG IS THE DYE STABLE AND HOW LONG CAN I MONITOR THE DYE UNDER THE MICROSCOPE?
1 answer-
This has not been determined. As with most fluorescent dyes, it will fade over time. See the link below. This reference may offer additional helpful information.
https://www.biorxiv.org/content/10.1101/2021.12.15.472742v1.full.pdfHelpful?
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