The signal from staining is generally maintained after formaldehyde fixation. However, prolonged storage beyond 24 hours may result in some signal attenuation, depending on storage conditions. For optimal retention, store samples at 2 to 8°C and protect them from light.
SCT119
BioTracker 650 Red Nuclear Dye
Live cell imaging red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Synonym(s):
Live cell imaging probe, Live cell nuclear probe
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Available to ship TODAYfromAreál Kühne+Nagel spol. s r.o. | CZK 10,200.00 |
About This Item
NACRES:
NA.47
UNSPSC Code:
12352207
CZK 10,200.00
Available to ship TODAYDetails
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
storage temp.
2-8°C
General description
The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell′s genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.
The BioTracker 650 Red Nuclear Dye is a cell-membrane permeable red fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, and it has low toxicity for live cell imaging. The dye is supplied with a vial of verapamil, an efflux pump inhibitor that may improve probe retention and live cell staining in certain cell types.
Spectral Properties
Absorbance: 650nm
Emission: 675nm
The BioTracker 650 Red Nuclear Dye is a cell-membrane permeable red fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, and it has low toxicity for live cell imaging. The dye is supplied with a vial of verapamil, an efflux pump inhibitor that may improve probe retention and live cell staining in certain cell types.
Spectral Properties
Absorbance: 650nm
Emission: 675nm
Application
Live cell fluorescent imaging
Live cell imaging red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Physical form
Liquid
Preparation Note
Store BioTracker 650 Red Nuclear Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Analysis Note
Spectral Properties
Absorbance: 650nm
Emission: 675nm
Absorbance: 650nm
Emission: 675nm
Other Notes
1) 1 vial of 50µL BioTracker 650 Red Nuclear Dye (1000X in DMSO) (CS224577)
2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)
2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. -10 to -25°C | storage temp. -10 to -25°C |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Is the signal maintained after formaldehyde fixation?
1 answer-
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Is there a risk of interference from phenol red in cell culture medium with the detection of this dye, as the protocol recommends dilution in cell culture medium?
1 answer-
Phenol red is unlikely to interfere with the detection of this dye. However, it's unnecessary to have phenol red in the cell culture medium at this point. Highly recommend using cell culture medium without phenol red.
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Is this dye compatible to image live migrating HL60 cells?
1 answer-
This product is suitable for live cell imaging. The HL60 cell line has not been specifically tested, however there is no reason to expect it will not work. While this stain is markedly more photostable that DAPI and other similar dyes, the retention time has not been determined. The addition of verapamil is recommended to improve retention time. See the link below for the product data sheet.
The protocol can be found on page 2:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/211/919/sct119ds.pdfHelpful?
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