PS1019

PhosphoDetect Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb

liquid, Calbiochem^®

• Synonym(s): Anti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr ^183/185), Anti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr^183/185)
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: mouse, rat, human
• Application: —
• Citations: 3

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: mouse, rat, human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (1:1000))
• isotype: IgG
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pThr183/pTyr185)
• Gene Information: human ... MAPK8(5599)

Description

General description

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 proteins phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵.

Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵ in uv-treated HEK293 cells.

This PhosphoDetect Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human JNK1 and JNK2

Application

Immunoblotting (1:1000)

Physical form

In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
UV-treated HEK293 cells or sorbitol-treated PC12 cells

Other Notes

Choudhury, B.K., et al. 2002. J. Immunol.169, 5955.
Barr, R.K., et al. 2001. Int. J. Biochem. Cell. Biol.33, 1047.
Dong, C., et al. 2001. J. Clin. Immunol.21, 253.
Janulis, M., et al. 2001. Mol. Cell. Biol.21, 2235.
Pearce, A.K., et al. 2001. Trends Cell Biol.11, 426.
Tong, T., et al. 2001. Exp. Cell Res.269, 64.
Cobb, M.H., et al. 1999. Prog. Biophys. Mol. Biol.71, 479.
Basu, S., et al. 1998. Oncogene17, 3277.

The immunogen sequence is highly conserved among many species, including mouse, rat, chicken, nematode, and Drosophila, but cross-reactivity has not been determined. The immunogen sequence is also conserved in JNK3, but reactivity has not been detected. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1