OB05

Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2)

liquid, clone 13/45/31/2, Calbiochem^®

• Synonym(s): Anti-His6
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 13/45/31/2, monoclonal
• Species reactivity: -
• Application: —
• Citations: 10

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 13/45/31/2, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity (predicted by homology): all
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (ELISA (0.1-0.4 µg/mL); Immunoblotting (1-4 µg/mL); Immunofluorescence (1-4 µg/mL); Immunoprecipitation (4 µg/mL))
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His•Tag sequence in native and recombinant proteins. Specifically recognizes an epitope of six consecutive His residues of both natural and recombinant sources.

Specifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources.

Immunogen

Epitope: HHHHHH

a recombinant protein containing a His•Tag sequence

Application

ELISA (0.1-0.4 g/ml)
Immunoblotting (1-4 g/ml)
Immunofluorescence (1-4 g/ml)
Immunoprecipitation (4 g/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Positive Control
Proteins containing a His•Tag sequence

Other Notes

Antibody should be titrated for optimal results in individual systems.

Zentgraf, H., et al. 1995. Nucl. Acids. Res.23, 3347.
Gu, J., et al. 1994. BioTechniques17, 257.
Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
Sisk, W.P., et al. 1994. J. Virol.68, 766.
Lu, T., et al. 1993. Anal. Biochem.213, 318.
Garner, J., et al. 1992. Cell69, 833.
Hochuli, E., et al. 1987. J. Chromatogr.411, 177.

Legal Information

Manufactured by Dianova, GmbH, Germany.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable