NB20

Anti-Ankyrin_G Mouse mAb (463)

liquid, ≥95% (SDS-PAGE), clone 463

• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 463, monoclonal
• Species reactivity: rat, mouse, human, canine
• Application: —
• Citations: 4

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 463, monoclonal
• assay: ≥95% (SDS-PAGE)
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: rat, mouse, human, canine
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Frozen Sections (1-2 µg/mL); Immunoblotting (2-5 µg/mL); Immunofluorescence (1-2 µg/mL))
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... ANK3(288)

Description

General description

Anti-Ankyrin G, mouse monoclonal, clone 463, recognizes the ~190 kDa ankyrin G protein in brain extracts. It is validated for Western blotting, immunofluorescence, and frozen sections.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of Ankyrin_G.

Recognizes the ~190 kDa ankyrin_G protein in brain extracts. Reacts with all spliced forms of ankyrin_G.

Antibody Target Gene Symbol: ANK3

Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049

Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)

Hu Entrez ID: 288 (Related Antibodies: AP1034, PC523, PC522, AP1057)

Mu Entrez ID: 11735

Rat Entrez ID: 361833

Immunogen

Human

a synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrin_G

Application

Frozen Sections (1-2 g/ml)
Immunoblotting (2-5 g/ml)
Immunofluorescence (1-2 g/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Positive Control
Mouse or rat brain extract or unmyelinated axons

Other Notes

Ankyrin_G is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.

Davis, J.Q., et al. 1995. Mol. Biol. Cell6, 177a, supplement.
Kunimoto, M., et al. 1995. FEBS Lett.357, 217.
Lambert, S., et al. 1995. Mol. Biol. Cell6, 98a supplement.
Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol.9, 27.
Kunimoto, M., et al. 1991. J. Cell Biol.115, 1319.
Otto, E., et al. 1991. J. Cell Biol.114, 241.
Davis, J., and Bennett, V. 1984. J. Biol. Chem.259, 13550.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable