MABS1966

Anti-PEG Antibody, clone 6.3

clone 6.3, from mouse

• Synonym(s): Polyethylene Glycol
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 6.3, monoclonal
• Technique(s): ELISA: suitable
• Application: ELISA
• Citations: -

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6.3, monoclonal
• species reactivity (predicted by homology): all
• packaging: antibody small pack of 25 μg
• technique(s): ELISA: suitable
• isotype: IgG1κ
• target post-translational modification: unmodified

Description

General description

Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. Clone 6.3 displays high affinity and binds the PEG backbone and can be employed as a detection antibody for the quantification of PEG. It binds to a smaller epitope of the PEG backbone when compared to other antibodies. Since it binds to a smaller PEG epitope (~750 Da), it is not compatible with detergents that contain an ethylene oxide backbone, such as Tween 20. Instead 0.05% CHAPS is a more suitable detergent when using this antibody.

Immunogen

PEG-conjugated to E.coli beta-glucuronidase.

Application

Anti-PEG, clone 6.3, Cat. No. MABS1966, is a mouse monoclonal antibody that detects PEG-conjugated proteins and it hs been tested for use in ELISA.

ELISA Analysis: A representative lot detected PEG in ELISA applications (Tung, H.Y., et. al. (2015). Mol Cancer Ther. 14(6):1317-26).

Research Category
Signaling

Biochem/physiol Actions

Clone 6.3 specifically detects proteins conjugated with polyethylene glycol (PEG).

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal antibody IgG1 in PBS with 0.04% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by ELISA in Immobilized PEG (10K-PEG, 3K-PEG, and 2K-mPEG).

ELISA Analysis: 5 µg/mL of Immobilized PEG (10K-PEG, 3K-PEG, and 2K-mPEG) were detected with this antibody.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.