MABF549

Anti-CD25 Antibody (mouse), PE-Cy7, clone PC61.5

clone PC61.5, 0.2 mg/mL, from rat

• Synonym(s): Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, CD25
• UNSPSC Code: 12352203
• NACRES: NA.44
• eCl@ss: 32160702
• Conjugate: PE-Cy7
• Clone: PC61.5, monoclonal
• Application: FACS, IHC, IP
• Citations: -

Properties

• biological source: rat
• Quality Level: 100
• conjugate: PE-Cy7
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: PC61.5, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• concentration: 0.2 mg/mL
• technique(s): flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
• isotype: IgG1λ
• UniProt accession no.: P01590
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Il2Ra(16184)

Description

General description

The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells.The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

This rat monoclonal antibody Anti-CD25 (mouse), PE-Cy7, clone PC61.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD25.

Physical form

Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Preparation Note

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 µg of this antibody detected CD25 in one million C57Bl/6 splenocytes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable