MABF360

Anti-CD117 (c-kit) Antibody (mouse), APC, clone ACK2

clone ACK2, 0.2 mg/mL, from rat

• Synonym(s): Mast/stem cell growth factor receptor Kit, SCFR, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: Allophycocyanin conjugate
• Clone: ACK2, monoclonal
• Application: FACS, ICC, IHC, WB
• Citations: -

Properties

• biological source: rat
• Quality Segment: 100
• conjugate: Allophycocyanin conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: ACK2, monoclonal
• species reactivity: mouse
• concentration: 0.2 mg/mL
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG2bκ
• UniProt accession no.: P05532
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Kit(16590)

Description

General description

CD117, also called c-Kit, is a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

This rat monoclonal antibody Anti-CD117 (c-kit) (mouse), APC & clone ACK2 validated for use in western blotting;IHC;ICC;Flow Cytometry for the detection of CD117 (c-kit).

Biochem/physiol Actions

This antibody is reported to be cross-reactive with rat CD117 and is extensively published for use in this species.

Physical form

Purified rat monoclonal antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Preparation Note

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 µg of this antibody detected CD117 in one million C57BI/6 bone marrow cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable