MABF2154

Anti-Ricin B Chain Antibody, clone RBC11

clone RBC11, from mouse

• Synonym(s): RBC
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: RBC11, monoclonal
• Application: ELISA, FACS
• Citations: -

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RBC11, monoclonal
• species reactivity: Ricinus communis
• packaging: antibody small pack of 25 μg
• technique(s): ELISA: suitable, flow cytometry: suitable
• isotype: IgG2bκ
• UniProt accession no.: D0VFC4
• target post-translational modification: unmodified

Description

General description

28.90 kDa calculated.

Ricin is a dimeric toxin derived from Ricinus communis (Caster bean). It is composed of a sugar-binding subunit (B chain) that attached to cell surface receptors and facilitate the entry of toxin into the target cell and a second subunit (A chain), which has glycosidase activity that inactivates ribosomes by depurinating a single adenosine residue from an exposed loop of the 28S ribosomal RNA. The toxic effects of Ricin are attributed to the A chain. Ricin B chain is shown to be a lectin that binds to galactosides found at the cell surface in a non-cooperative manner. It has two Ricin-B type lectin domains (aa 7-134 and 137-261). The mode of galactose binding is reported to be nearly identical at the two sites. Clone RBC11 is shown to block binding of Ricin to the cell. (Ref.: Maddaloni, M., et al. (2004). J. Immunol. 172; 6221-6228; Rutenber, E., et al. (1987). Nature. 326(6113):624-626).

Immunogen

Purified Ricin B chain.

Application

Anti-Ricin B Chain, clone RBC11, Cat. No. MABF2154, is a mouse monoclonal antibody that detects Ricin B chain and has been tested for use in ELISA and Flow Cytometry.

Flow Cytometry Analysis: A representative lot detected Ricin B chain in Flow Cytometry applications (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8).

ELISA Analysis: A representative lot detected Ricin B chain in ELISA applications (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8; Simon, S., et. al. (2015). Toxins (Basel) 7(12):4967-86).

Research Category
Inflammation & Immunology

Biochem/physiol Actions

Clone RBC 11 binds to Ricin B chain.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by ELISA with Ricin B chain.

ELISA Analysis: Various dilutions of this antibody detected Ricin B chain in an indirect ELISA.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk: WGK 1
• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable