MABE268

Anti-Musashi-1 Antibody, clone 7B11.1

clone 7B11.1, from mouse

• Synonym(s): RNA-binding protein Musashi homolog 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 7B11.1, monoclonal
• Application: IHC, WB
• Citations: 5

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 7B11.1, monoclonal
• species reactivity: rat, mouse, human
• technique(s): immunohistochemistry: suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_002433
• UniProt accession no.: O43347
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MSI1(4440)

Description

General description

In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, cellular differentiation, nuclear signaling and tumorigenesis. Musashi-1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi-1 is a selective marker for intestinal stem or early lineage cells. Musashi-1 interacts with the Notch pathway during asymmetric cell division by binding the 3′ UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi-1 is present.

~39 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to human Musashi-1.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Neural Stem Cells

Use Anti-Musashi-1 Antibody, clone 7B11.1 (Mouse Monoclonal Antibody) validated in WB, IHC to detect Musashi-1 also known as RNA-binding protein Musashi homolog 1.

Western Blot Analysis: A representative lot detected Musashi-1 in HEK293, NIH/3T3, and PC-12 cell lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Musashi-1 in mouse cerebellum, normal mouse brain, and normal human pons tissue.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blot in mouse E17 spinal cord tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Musashi-1 in 10 µg of mouse E17 spinal cord tissue lysate.

Control
Mouse E17 spinal cord tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 03-114; 04-1041

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable