MABC008

Mouse IgM Negative Control, clone GC323

Mouse IgM Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

• UNSPSC Code: 12352203
• NACRES: NA.42
• eCl@ss: 32160702
• Clone: GC323, monoclonal
• Technique(s): flow cytometry: suitable, immunofluorescence: suitable
• Application: FACS, IF
• Citations: 4

Properties

• biological source: mouse
• Quality Segment: 100
• clone: GC323, monoclonal
• manufacturer/tradename: Chemicon^®
• concentration: 0.5 mg/mL
• technique(s): flow cytometry: suitable, immunofluorescence: suitable
• isotype: IgM
• shipped in: wet ice
• target post-translational modification: unmodified

Description

Immunogen

Protein I preparation of Neisseria gonorrhoeae.

Application

Research Category
Secondary & Control Antibodies

Epitope Tags & General Use

Research Sub Category
Isotype Control Antibodies

The use of this antibody enables an estimation of non-specific binding of mouse monoclonal antibodies of isotype IgM to cell surface components in human blood and bone marrow. It is not suitable for use in immunohistochemical procedures.

SUGGESTED USAGE

The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used. Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

Biochem/physiol Actions

This antibody reacts specifically with the outer membrane protein I component of various strains of Neisseria gonorrhoeae and does not react with any human cell surface component.

Physical form

Format: Purified

Purified from mouse ascitic fluid. The antibody is supplied in 0.5ml 25mM Tris HCl, 0.4M NaCl, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis, specific immunofluorescence assay and flow cytometry.

Preparation Note

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable