MAB4307

Anti-Mouse Leukemia Inhibitory Factor Antibody, clone 2H2.2

clone 2H2.2, Chemicon^®, from mouse

• Synonym(s): mLIF
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 2H2.2, monoclonal
• Application: ELISA, WB
• Citations: 4

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2H2.2, monoclonal
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, western blot: suitable
• input: sample type hematopoietic stem cell(s); sample type neural stem cell(s)
• isotype: IgG1
• UniProt accession no.: P09056, P15018
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... LIF(3976)

Description

General description

Leukemia inhibitory factor, or LIF, is a pleiotropic cytokine that is expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. Human LIF cDNA encodes a 202 amino acid precursor which is processed to yield a 180 amino acid, biologically active glycoprotein with an approximate molecular weight of 38-67 kDa. In addition to its involvement in the stimulation of acute-phase protein during inflammatory processes, LIF has also been implicated in promoting the survival and growth of axons in vitro. LIF signals through the LIF receptor, a heterodimer comprised of a 190 kDa LIFR subunit and the gp130 subunit common to the IL-6, IL-11, CNTF and OSM receptors. Janus tyrosine kinases, JAK1 and JAK2, are activated in response to LIF stimulation, which in turn phosphorylate Stat1 and Stat3 causing their nuclear translocation and the transcription of Stat-responsive genes.

Immunogen

Recombinant Murine LIF.

Application

Anti-Mouse Leukemia Inhibitory Factor Antibody, clone 2H2.2 is an antibody against Mouse Leukemia Inhibitory Factor for use in ELISA & WB.

Immunoblotting: a starting dilution of 1-10 μg/mL is suggested.

EIA

Optimal working dilutions must be determined by the end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Biochem/physiol Actions

This antibody is reactive to E.coli and STO derived recombinant murine Leukemia Inhibitory Factor (LIF).

Physical form

Format: Purified

Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl ph 7.6 with 0.1% Sodium Azide.

Preparation Note

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Analysis Note

Control
Mouse DA-1a cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Storage Class: 12 - Non Combustible Liquids