MAB3540

Anti-Lamin A Antibody, CT, a.a. 598-611, clone 133A2

clone 133A2, Chemicon^®, from mouse

• Synonym(s): 70 kDa Lamin
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 133A2, monoclonal
• Application: FACS, ICC, IHC, WB
• Citations: 14

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 133A2, monoclonal
• species reactivity: rat, mouse, canine, bovine, human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG3
• NCBI accession no.: NM_005572.3, NM_170707.2, NM_170708.2
• UniProt accession no.: P02545
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... LMNA(4000)

Description

General description

Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

Immunogen

Epitope: C-terminus, a.a. 598-611

Synthetic peptide from the C-terminus of human lamin A. This sequence is not present in the the lamin C isoform.

Application

Immunoblotting: Lamin A detected as a 70kDa protein under reduced conditions.

Immunocytochemistry on fixed cells (methanol/acetone fixation)

Immunohistochemistry on frozen tissue sections.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Lamin A Antibody, C-terminus, a.a. 598-611, clone 133A2 is validated for use in FC, WB, IC, IH for the detection of Lamin A.

Biochem/physiol Actions

Monoclonal 133A2 reacts against human lamin A but not lamin C. The epitope lies with the 98 amino acids of the C terminus that is unique to lamin A. Specifically deletion analysis has shown that amino acids 598-611 were essential for reactivity {Hozak, P et.al. (1995), J. Cell Sci 635-644}.

Physical form

Format: Purified

Liquid in buffer containing 0.1% sodium azide.

Preparation Note

Maintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable