MAB3446

Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9

ascites fluid, clone 2HP-2G9, Chemicon^®

• Synonym(s): HP1a, Antigen p25, Chromobox Protein Homolog 5
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 2HP-2G9, monoclonal
• Application: ELISA, IP, WB
• Citations: 20

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 2HP-2G9, monoclonal
• species reactivity: mouse, human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_012117.1
• UniProt accession no.: P45973
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CBX5(23468)

Description

General description

25 kDa

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.

Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Immunogen

Recombinant mouse HP1alpha.

Application

Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5 & has been validated in ELISA, IP & WB.

ELISA: 1:500-1:5,000

Western blot: 1:500-1:5,000

Immunoprecipitation: 1:500-1:5,000

Immunohistochemistry/Immunocytochemistry: Not Recommended

Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.

Physical form

Liquid ascites fluid containing no preservative.

Analysis Note

Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable