MAB3402

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5

clone GA5, Chemicon^®, from mouse

• Synonym(s): GFAP
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: GA5, monoclonal
• Application: ICC, IHC (p), WB
• Citations: 414

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: GA5, monoclonal
• species reactivity: chicken, pig, rat, bovine, mouse, rabbit, human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG1
• UniProt accession no.: F1RR02
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... GFAP(2670)

Description

General description

50 kDa

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

Immunogen

Purified Glial Fibrillary Acidic Protein (GFAP) from pig spinal cord (Debus, E., 1983).

Application

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 is an antibody against Glial Fibrillary Acidic Protein for use in IC, IH, IH(P) & WB with more than 55 product citations.

Immunocytochemistry:
5 μg/mL of a previous lot was used.

Immunohistochemistry:
5 µg/mL

Immunoblotting:
Recognizes a 51kDa protein in reducing westerns of total brain lysates.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Biochem/physiol Actions

Expected to cross-react with porcine, chicken, bovine, and rabbit.

The antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.

Preparation Note

Store the reconstituted antibody at 2-8°C for up to 6 months after date of receipt.
DO NOT FREEZE.

Analysis Note

Control
Mouse brain tissue, Astrocyte culture.

Routinely evaluated by Western Blot on Mouse brain lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected Glial Fibrillary Acidic Protein on 10 μg of Mouse Brain lysate.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Replaces: 04-1031; 04-1062

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2