MAB3232

Anti-Cytokeratin 14 Antibody, clone RCK107

clone RCK107, Chemicon^®, from mouse

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: RCK107, monoclonal
• Application: FACS, ICC, IHC, WB
• Citations: 17

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RCK107, monoclonal
• species reactivity: rat, pig, human, canine
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG3
• NCBI accession no.: NM_000526.3
• UniProt accession no.: P02533
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... KRT14(3861) ; rat ... Krt14(287701)

Description

General description

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.

Immunogen

Cytoskeletal preparation of TR146 epithelial cells.

Application

Detect Cytokeratin 14 using this Anti-Cytokeratin 14 Antibody, clone RCK107 validated for use in FC, WB, IC, IH.

Research Category
Cell Structure

Research Sub Category
Cytokeratins

Western blot

Immunohistochemistry on frozen tissue sections.

Immunocytochemistry

Flow cytometry

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Cytokeratin 14. MAB3232 reacts with basal cell compartments of stratified and combined epithelia.Reacts with cytokeratin 14, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands.

Physical form

Format: Purified

Liquid in buffer with 0.1% sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable