MAB201P

Mouse Anti-Rabbit light chain, HRP conjugate Antibody

Chemicon^®, from mouse

• Synonym(s): HRP Anti-Rabbit, Light Chain Detection Antibody, Rabbit Light Chain Detection
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: peroxidase conjugate
• Clone: monoclonal
• Application: ELISA, ICC, IHC, WB
• Citations: 30

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: peroxidase conjugate
• antibody form: purified antibody
• antibody product type: secondary antibodies
• clone: monoclonal
• species reactivity: rabbit
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

25 kDa

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Immunogen

Epitope: Kappa light chain

Prepared from rabbit IgG light chain.

Application

Detect Rabbit light chain using this Mouse anti-Rabbit light chain, HRP conjugate Antibody validated for use in ELISA, WB, IC, IH.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

Western Blotting: 1:5,000 -1:100,000 with chromogenic substrates 1:10,000 -1:200,000 with ECL substrates
ELISA: 1:5,000 -1:100,000
Immunohistochemistry: 1:500 - 1:5,000
Immunocytochemistry: 1:500 - 1:5,000

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep.

The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rabbit IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep immunoglobulins.

Physical form

Purified from antiserum by chromatographic procedures. Lyophilized in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. 15 mg/mL BSA as stabilizer.

Serum

Preparation Note

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2