IPVH85R

Immobilon^®-P Membrane

1 roll, 8.5 cm x 10 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

• Synonym(s): Immobilon^® NOW, Immobilon^® narrow roll, Western blotting membrane, blotting membrane, transfer membrane
• UNSPSC Code: 41105339
• eCl@ss: 32031613
• NACRES: NB.23
• Material: PVDF membrane, plain filter, white filter
• Detection method: chemiluminescent, colorimetric, fluorometric, radioactive
• Pore size: 0.45 μm pore size
• Compatibility: for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

Properties

• Product Name: Immobilon^®-P Membrane, PVDF, 0.45 m, 8.5 cm x 10 m roll, 0.45 um ( 0.45 micron filter ) pore size Hydrophobic immobilon-P PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.
• Quality Segment: 400
• material: PVDF membrane, plain filter, white filter
• feature: hydrophobic
• manufacturer/tradename: Immobilon^®
• technique(s): dot blot: suitable, western blot: suitable
• filter L × W: 8.5 cm × 10 m
• pore size: 0.45 μm pore size
• capacity: 160 μg/cm² adsorption capacity (insulin), 215 μg/cm² adsorption capacity (BSA), 294 μg/cm² adsorption capacity (goat IgG)
• compatibility: for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination
• detection method: chemiluminescent, colorimetric, fluorometric, radioactive
• shipped in: ambient

Description

General description

Immobilon^®-P transfer membrane is a hydrophobic polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 micron (μm). It is used for the transfer of proteins from a variety of gel matrices. Immobilon^®-P transfer membrane is recommended for most western blotting, especially proteins greater than 20 kDa. This membrane displays improved handling characteristics and staining capabilities, a higher signal-to-noise ratio for enhanced sensitivities, and improved solvent resistance, when compared to nitrocellulose membrane.
Primary Binding Mechanisms: Electrostatic, hydrophobic,
Filter Code: IPVH

Application

Immobilon^®-P Membrane, PVDF, 0.45 μm, 8.5 cm x 10 m roll has been used in western blot analysis[1][2] and co-immunoprecipitation.[2]

Other Notes

Replaces: INCP00010

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany